Abstract
Angiogenesis is a cascade-like mechanism, essential for the progression of solid tumours. Quantifying microvessel density is considered the gold standard for measuring baseline angiogenesis and indeed "the response to intervention". Applying immunohistochemical techniques to biopsies conventionally does this; however, it would be very helpful to have a less invasive, reliable method of quantifying blood flux, and hence angiogenesis. The aim of the study was to validate the use of a laser Doppler flux meter as an experimental tool in laryngology. The principle and technique of laser Doppler flux-metry is described. A validation study designed to estimate the reproducibility and ability to measure change was performed in the human larynx and in an animal model. Statistical advice was sought and analysis was performed using SPSS software. Our experiments show good reproducibility of laser Doppler measurements in the human and animal model (correlation coefficient 0.956 and 0.947, respectively, P =0.01). We have also shown that the laser Doppler flux meter is capable of detecting change when it is expected ( P <0.01). Utilising a standardised technique, laser Doppler flux-metry appears to be a reliable and reproducible method of estimating blood flux in the larynx.
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Acknowledgements
This study was made possible by funds kindly donated from the estate of the late Thomas Green Esquire and managed by the Medway Head and Neck Cancer Research Fund. The authors acknowledge the kind advice provided by Mr Colin Cryer, statistician at the University of Kent. The kind advice and guidance provided by Professor Michael Gleeson, Guys Hospital, London, is gratefully acknowledged.
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Jacob, A., Birchall, M.A. Laser Doppler flux-metry as an experimental tool in laryngology. Eur Arch Otorhinolaryngol 260, 308–311 (2003). https://doi.org/10.1007/s00405-002-0575-6
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DOI: https://doi.org/10.1007/s00405-002-0575-6