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The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study is to evaluate the sperm DNA fragmentation index (DFI) in oocyte donation cycles and correlate it with the sperm parameters, the male characteristics, the embryo quality and the outcome of intracytoplasmic sperm injection (ICSI).

Methods

A total of 150 couples participating in an oocyte donation program were included in the study. Sperm samples were assessed by conventional sperm analysis. DFI was evaluated using the Halosperm kit, a sperm chromatin dispersion test (SCD).

Results

The relations between DNA damage and epidemiological male factors (age, height, weight), standard semen parameters (concentration, total and forward motility, and morphology), and embryological and clinical parameters (fertilization rate, total blastocyst number, number of good quality blastocyst, clinical pregnancy) were analyzed. DFI was positively correlated with advanced male age (r = 0.23, p < 0.05) and negatively correlated with total sperm and forward motility (r = − 0.29, r = − 0.27, respectively; p < 0.05). DFI was not significantly correlated with pregnancy outcome in oocyte donation cycles (r = − 0.05, p > 0.05). When good quality blastocysts were chosen, a trend toward the development of good quality embryos was detected in the presence of a low DFI (r = − 0.20, p = 0.08).

Conclusions

DFI does not significantly affect the outcome of ICSI in oocyte donation cycles. Even in cases of advanced paternal age that a high DFI resulted sperm DNA fragmentation seems not to adversely affect the final outcome.

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Acknowledgements

Preliminary data regarding this study were presented as poster at the 10th European Congress of Andrology, October 2018 in Budapest, Hungary.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Life, Health and Environmental Sciences, University of L’Aquila, Via Vetoio, Coppito 2, 67100, L’Aquila, Italy

    Sevastiani Antonouli, Maria Grazia Palmerini & Guido Macchiarelli

  2. Iakentro Fertility Center, IVF Laboratory, 45250, Thessaloniki, Greece

    Achilleas Papatheodorou, Yannis Panagiotidis, Stamatios Petousis, Nikos Prapas & Yannis Prapas

  3. Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Laboratory of Electron Microscopy “Pietro M. Motta”, University La Sapienza, 00161, Rome, Italy

    Stefania Annarita Nottola

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  1. Sevastiani Antonouli
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Contributions

SA: Data collection or management, data analysis, manuscript writing. AP: Protocol/project development, data analysis, manuscript writing/editing. YPa: Protocol/project development, data analysis. SP: Data management, data analysis. NP: Data management, data analysis, manuscript editing. SAN: Data analysis, manuscript editing. MGP: Data collection or management. GM: Data analysis, manuscript editing. YPr: Protocol/project development, data management, data analysis, manuscript writing/editing.

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Correspondence to Sevastiani Antonouli.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee, and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The Institutional Review Board of Iakentro Advanced Medical Center/ethics committee of Thessaloniki, Greece approved the study at 2/9/2013, Ref. Number 9/2013.

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Antonouli, S., Papatheodorou, A., Panagiotidis, Y. et al. The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes. Arch Gynecol Obstet 300, 207–215 (2019). https://doi.org/10.1007/s00404-019-05133-9

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