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Invariant natural killer T cells are reduced in peripheral blood of bullous pemphigoid patients and accumulate in lesional skin

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Abstract

iNKT (invariant natural killer T) cells are unconventional immunoregulatory T cells which contribute to B cell maturation, antibody and cytokine production. iNKT cells are implicated in the control of autoimmune inflammation in different disorders. For bullous pemphigoid (BP), the most frequent bullous autoimmune dermatosis, the role of iNKT cells has not yet been studied. We, therefore, aimed at investigating the frequency of iNKT cells in peripheral blood and biopsies from lesional and non-lesional skin from patients with BP and controls. Circulating CD3+iTCR+ iNKT cells were assessed by flow cytometry in peripheral blood from 30 patients with BP and from 29 controls (19 patients with skin tumors and 10 healthy controls). In 34 lesional and 13 non-lesional skin biopsies from BP patients and 17 biopsies from control individuals the number of Vα24+Vβ11+ iNKT cells was investigated by immunofluorescence staining. BP patients showed a significantly lower frequency of circulating iNKT cells compared to the control group. Patients with severe disseminated blistering tended to display lower iNKT cell numbers than patients with moderate disease severity. In lesional skin of BP patients, an enrichment of iNKT cells was detected compared to skin biopsies from controls. Similarly to control biopsies, non-lesional biopsies of BP patients contained only few iNKT cells. In conclusion, the deficiency of circulating iNKT cells associated with enrichment at the site of cutaneous inflammation suggests that iNKT cells may play a pathophysiologically relevant role in BP.

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Acknowledgements

This work was supported by HELIOS Research Support, ID 002342, to S.C. Hofmann. The authors thank Berit Geis and Dr. Frank Krummenauer, Institute for Medical Biometry and Epidemiology, Faculty of Health, University Witten/Herdecke, for help with the statistical analysis.

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  1. Linda M. Mathias and Miriam Harff contributed equally.

Authors and Affiliations

  1. Department of Dermatology, Allergology and Dermatosurgery, HELIOS University Hospital Wuppertal, University Witten/Herdecke, Heusnerstr. 40, 42283, Wuppertal, Germany

    Linda M. Mathias, Miriam Harff & Silke C. Hofmann

  2. Department of Surgery, HELIOS University Hospital Wuppertal, University Witten/Herdecke, Wuppertal, Germany

    Valerie Orth

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  1. Linda M. Mathias
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  2. Miriam Harff
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  3. Valerie Orth
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  4. Silke C. Hofmann
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Correspondence to Silke C. Hofmann.

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Patients provided informed consent and ethical approval was obtained from the University Witten/Herdecke ethics committee.

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Mathias, L.M., Harff, M., Orth, V. et al. Invariant natural killer T cells are reduced in peripheral blood of bullous pemphigoid patients and accumulate in lesional skin. Arch Dermatol Res 312, 747–751 (2020). https://doi.org/10.1007/s00403-019-02029-1

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  • DOI: https://doi.org/10.1007/s00403-019-02029-1

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