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Growth factor expression after supraspinatus tear: a quantitative polymerase chain reaction (PCR) study in rats

  • Arthroscopy and Sports Medicine
  • Published:
Archives of Orthopaedic and Trauma Surgery Aims and scope Submit manuscript

Abstract

Introduction

The objective of this study was to evaluate the temporal expression pattern of three different growth factors (VEGF, IL-1β, and TGF-1β) in a supraspinatus tendon lesion in an animal model. The hypothesis of this study is that there are variations in the expression of these factors in the first 8 weeks after injury.

Materials and methods

A full thickness defect was made in the supraspinatus tendon of 40 rat shoulders. The animal were sacrificed at 0, 3, 7, 14 and 56 days after injury and three tissue samples were obtained: bone from the tendon footprint; the supraspinatus tendon stump, and a fragment of the myotendinous junction. After mRNA extraction, quantitative PCR analysis was performed, and the expression of three different growth factors were evaluated in each zone.

Results

There was an increased expression of IL-1β during the first week after injury at all levels evaluated with a clear peak in the first day after injury. There was also a significant increase in TGF-1β expression levels all along the first week in the three zones. There were no variations in VEGF expression in the three zones along the 8 weeks.

Conclusion

IL-1β was expressed predominantly in the initial stages after injury; TGF initiated its expression after the initial phase since day three, whereas VEGF remained basically unchanged during the entire process.

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Acknowledgments

Approved by Dña Ana Isabel Ortiz Chercoles, Presidenta Comité Bienestar Animal (CEBA) Hospital Ramón y Cajal Madrid Spain.

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Authors and Affiliations

  1. Unidad de Hombro y Codo, Servicio de Traumatología y Cirugía Ortopédica, Hospital Universitario Ramón y Cajal, Madrid, Spain

    Jorge Díaz Heredia, M. A. Ruiz Iban & R. Ruiz Díaz

  2. Servicio de Bioquímica-Investigación, Hospital Universitario Ramón y Cajal, IRYCIS, Madrid, Spain

    J. Martínez-Botas

  3. CIBER de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain

    J. Martínez-Botas

  4. Servicio de Traumatología y Cirugía Ortopédica, Fundación Jiménez Díaz, Madrid, Spain

    M. Valencia Mora

  5. Servicio de Traumatología y Cirugía Ortopédica, Hospital Galdakao Usansolo, Galdakao, Spain

    A. Cuéllar Ayestaran

  6. Servicio de Traumatología y Cirugía Ortopédica, Hospital MAZ, Zaragoza, Spain

    S. Moros Marco

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  1. Jorge Díaz Heredia
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Correspondence to Jorge Díaz Heredia.

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Díaz Heredia, J., Ruiz Iban, M.A., Martínez-Botas, J. et al. Growth factor expression after supraspinatus tear: a quantitative polymerase chain reaction (PCR) study in rats. Arch Orthop Trauma Surg 136, 1563–1570 (2016). https://doi.org/10.1007/s00402-016-2518-5

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