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Tunable immobilization of protein in anionic spherical polyelectrolyte brushes as observed by small-angle X-ray scattering

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Abstract

Tunable immobilization of bovine serum albumins (BSA) onto anionic spherical polyelectrolyte brushes (SPB) by changing BSA concentration, pH, and ionic strength was mainly observed by small-angle X-ray scattering (SAXS). Change of the BSA amount immobilized in SPB can be determined by SAXS which was confirmed by UV spectroscopy, and SAXS is the unique method to “see” the distribution of BSA in SPB. More BSA entered into brush layer upon increasing the protein concentration or decreasing the ionic strength of solutions. When pH increased from 3 to 5 (around the isoelectric point of BSA 4.9), more BSA came into the brush inner layer, while the proteins partly moved to the outer layer when pH continued to increase. After pH was higher than 7, most of BSA were desorbed from SPB. SAXS is proved to be a powerful tool to monitor the tunable immobilization and distribution of proteins in SPB.

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Acknowledgments

We thank Prof. Matthias Ballauff, Prof. Yan Lu, and Dr. Katja Henzler for their very helpful discussion on this work. The support by the NSFC Grants (51273063 and 21476143), the Fundamental Research Funds for the Central Universities, the high school specialized research fund for the doctoral program (20110074110003), 111 Project Grant (B08021), Shanghai Synchrotron Radiation Facility, European Synchrotron Radiation Facility, and the China Scholarship Council are gratefully acknowledged.

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Authors and Affiliations

  1. State-Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Weihua Wang, Li Li, Haoya Han, Yuchuan Tian, Zhiming Zhou & Xuhong Guo

  2. Engineering Research Center of Xinjiang Bingtuan of Materials-oriented Chemical Engineering, Shihezi University, Shihezi, Xinjiang, 832000, People’s Republic of China

    Xuhong Guo

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  1. Weihua Wang
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  2. Li Li
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Correspondence to Li Li or Xuhong Guo.

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Wang, W., Li, L., Han, H. et al. Tunable immobilization of protein in anionic spherical polyelectrolyte brushes as observed by small-angle X-ray scattering. Colloid Polym Sci 293, 2789–2798 (2015). https://doi.org/10.1007/s00396-015-3684-7

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