Skip to main content
SpringerLink
Log in

Effects of NaOH treatment of cereal starch granules on the extent of granular starch hydrolysis

  • Original Contribution
  • Published:
Colloid and Polymer Science Aims and scope Submit manuscript

Abstract

Effect of NaOH treatment on granular hydrolysis of cereal starches was studied and granular starch hydrolyzing enzyme is used to hydrolyze native and NaOH-treated starch for 24 h. The dextrose equivalent value of NaOH-treated starch increased significantly compared to native starch, i.e., 28–38 % for corn, 7–37 % for rice, but no significant increase for corn starch. Scanning electron microscopy micrographs showed that NaOH treatment caused an enlargement of pores and degrades the surface of starch granules. Hydrolyzed-treated starch exhibited rougher surface and more porous granules compared to native starch. The swelling power and pasting properties of NaOH-treated starches were markedly altered after hydrolysis. X-ray pattern of all starches showed no changes and the amylose content decrease significantly after hydrolysis, which could due to extensive degradation of amorphous region. Evidently, NaOH treatment below gelatinization temperature was effective in enhancing the degree of granular starch hydrolysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Zhang T, Oates CG (1999) Relationship between α-amylase degradation and physico-chemical properties of sweet potato starches. Food Chem 65:157–163

    Article  Google Scholar 

  2. Morrison WR (1981) Starch-lipids: a reappraisal. Starch-Starke 33:408–410

    Article  CAS  Google Scholar 

  3. Hoover R, Manuel H (1996) The effect of heat-moisture treatment on the structure and physicochemical properties of normal maize, waxy maize, dull waxy maize and amylomaize V starches. J Cereal Sci 23:153–162

    Article  CAS  Google Scholar 

  4. Vasanthan T, Sosulski FW, Hoover R (1995) The reactivity of native and autoclaved starches from different origins towards acetylation and cationization. Starch 4:135–143

    Article  Google Scholar 

  5. Guilbot, A. and Mercier, C. (1985). Starch. In: The Polysaccharides. USA: Academic Press. Vol 3. pp. 256–261

  6. Oates CG (1997) Towards an understanding of starch granule structure and hydrolysis. Food Sci Technol 8:375–382

    Article  CAS  Google Scholar 

  7. Vasanthan T, Hoover R (1992) Effect of defatting on starch structure and physicochemical properties. Food Chem 45:337–347

    Article  CAS  Google Scholar 

  8. Leloup VM, Colonna P, Buleon A (1991) Influence of amylose-amylopectin ratio on gel properties. J Cereal Sci 16:253–266

    Article  Google Scholar 

  9. Mistry AH, Eckhoff AR (1992) Characteristics of alkali-extracted starch obtained from corn flour. Cereal Chem 69:296–303

    CAS  Google Scholar 

  10. Medcalf DG, Gilles KA (1966) Effect of a lyotropic on series on the pasting characteristics of wheat and corn starches. Starch-Starke 18:101–105

    Article  CAS  Google Scholar 

  11. Lamsal BP, Wang H, Johnson LA (2011) Effect of corn preparation methods on dry-grind ethanol production by granular starch hydrolysis and partitioning of spent beer solids. Bioresour Technol, in press

  12. Wang P, Singh V, Xue H, Johnston DB, Rausch KD, Tumbleson ME (2006) Comparison of raw starch hydrolyzing enzyme with conventional liquefaction and saccharification enzymes in dry-grind corn processing. Cereal Chem 84:10–14

    Article  Google Scholar 

  13. Liukkonen KH, Montfoort A, Laakso SV (1992) Water-induced lipid changes in oat processing. J Agric Food Chem 40:126–130

    Article  CAS  Google Scholar 

  14. Wang L, Wang YJ (2001) Structures and physicochemical properties of acid- thinned corn, potato and rice starches. Starch-Starke 53:570–576

    Article  CAS  Google Scholar 

  15. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  16. Lauro M, Forssell PM, Suortti MT, Hulleman SHD, Poutanen KS (1999) α-amylolysis of large barley starch granules. Cereal Chem 76(6):925–930

    Article  CAS  Google Scholar 

  17. McGrance SJ, Cornell HJ, Rix CJ (1998) A simple and rapid colorimetric method for the determination of amylose in starch products. Starch-Starke 50:158–163

    Article  CAS  Google Scholar 

  18. Schoch TJ (1964) Swelling power and solubility of granular starches. In: Whistler RL (ed) Methods in carbohydrate chemistry, vol. 4. Academic Press, London

    Google Scholar 

  19. Wootton M, Ho P, Kensington NSW (1989) Alkali gelatinization of wheat starch. Starch-Starke 41:261–265

    Article  CAS  Google Scholar 

  20. Han XZ, Hamaker BR (2002) Association of starch granule proteins with starch ghost and remnants revealed by confocal laser scanning microscopy. Cereal Chem 79:892–896

    Article  CAS  Google Scholar 

  21. French D (1984) Organization of starch granules. In: Whistler RL, BeMiller JN, Paschall EF (eds) Starch: Chemistry and technology. Academic Press, Inc, USA, pp 184–247

    Google Scholar 

  22. Chen J, Jane J (1994) Properties of granular cold-water-soluble starches prepared by alcoholic-alkaline treatments. Cereal Chem 71(6):623–626

    CAS  Google Scholar 

  23. Wang YJ, Wang L (2003) Physicochemical properties of common and waxy corn starches oxidized by different levels of sodium hypochlorite. Carbohydr Polym 52:207–217

    CAS  Google Scholar 

  24. Fannon JE, Hauber RJ, BeMiller JN (1992) Surface pores of starch granules. Cereal Chem 69(3):284–288

    Google Scholar 

  25. Fannon JE, Shull JM, BeMiller JN (1993) Interior channels of starch granules. Cereal Chem 70(5):611–613

    Google Scholar 

  26. Helbert W, Schulein M, Henrissat B (1996) Electron microscopic investigation of the diffusion of Bacillus licheniformis alpha-amylase into corn starch granules. Int J Biol Macromol 19:165–169

    Article  CAS  Google Scholar 

  27. Tester RF, Morrison WR (1990) Swelling and gelatinization of cereal starches. I. Effects of amylopectin, amylose, and lipid. Cereal Chem 67:551–557

    CAS  Google Scholar 

  28. Morrison WR, Tester RF, Snape CE, Law R, Gidley MJ (1993) Swelling and gelatinization of cereal starches. IV. Some effects of lipid-complexed amylose and free amylose in waxy and normal barley starches. Cereal Chem 70:385–391

    CAS  Google Scholar 

  29. Hamaker BR, Griffin VK (1993) Effect of disulfide bond-containing protein on rice starch gelatinization and pasting. Cereal Chem 70:377–380

    CAS  Google Scholar 

  30. Ragheb AA, El-Thalouth IA, Tawfik S (1995) Gelatinization of starch in aqueous alkaline solutions. Starch-Starke 46:338–345

    Article  Google Scholar 

  31. Meyer KH, Bernfeld P, Boissonnas RA, Gurtler P, Noelting G (1949) Starch solutions and pastes and their molecular interpretation. J Phys Chem 53:319–334

    Article  CAS  Google Scholar 

  32. Beta T, Corke H, Taylor JRN, Rooney LW (2001) Effect of steeping treatment on pasting and thermal properties of sorghum starches. Cereal Chem 78(3):303–306

    Article  CAS  Google Scholar 

  33. Ganz AJ (1965) Effect of sodium chloride on the pasting of wheat starch granules. Cereal Chem 42:429–431

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Industrial Technology, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    U. Uthumporn, Y. N. Shariffa, A. Fazilah & A. A. Karim

Authors
  1. U. Uthumporn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. N. Shariffa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Fazilah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Karim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to U. Uthumporn.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Uthumporn, U., Shariffa, Y.N., Fazilah, A. et al. Effects of NaOH treatment of cereal starch granules on the extent of granular starch hydrolysis. Colloid Polym Sci 290, 1481–1491 (2012). https://doi.org/10.1007/s00396-012-2674-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00396-012-2674-2

Keywords

Search

Navigation