Abstract
A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(α,γ,l-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5–45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nanoparticles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV–Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.
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Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Desai Lab at UCSF is also acknowledged for support and NIH grant K99-DE021416. Confocal microscopy data for this study were acquired at the Nikon Imaging Center at UCSF.
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Stevanović, M., Savanović, I., Uskoković, V. et al. A new, simple, green, and one-pot four-component synthesis of bare and poly(α,γ,l-glutamic acid)-capped silver nanoparticles. Colloid Polym Sci 290, 221–231 (2012). https://doi.org/10.1007/s00396-011-2540-7
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DOI: https://doi.org/10.1007/s00396-011-2540-7