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Tart cherry (Prunus cerasus L.) dietary supplement modulates visceral adipose tissue CB1 mRNA levels along with other adipogenesis-related genes in rat models of diet-induced obesity

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Abstract

Purpose

There is increasing evidence for the involvement of dietary bioactive compounds in the cross-talk modulation of endocannabinoid system and some of the key regulators of transcriptional control for adipogenesis.

Methods

We aimed to characterize the expression of cannabinoid CB1/CB2 receptors and fatty acid amide hydrolase (FAAH) along with selected adipogenesis-related genes (PPARγ, SREBP-1c and PREF-1), adipocyte-secreted factors (leptin and adiponectin), mitochondrial bioenergetic modulators (PGC-1A and UCP-2), and transient receptor potential vanilloid subtype 1 (TRPV1) and 2 (TRPV2) channels in visceral adipose tissue of rats fed with a high-fat diet (HFD) containing either tart cherry seeds alone or tart cherry seeds and juice for 17 weeks. The visceral adipose tissue was weighed and checked the expression of different markers by qRT-PCR, Western blot and immunohistochemistry.

Results

Tart cherry supplements were able to downregulate the HFD-induced mRNA expression of CB1 receptor, SREBP-1c, PPARγ, leptin, TRPV1 and TRPV2 resulting in potential anti-adipogenic effects.

Conclusion

The present study points out that the intake of bioactive constituents of tart cherry may attenuate the effect of adipogenesis by acting directly on the adipose tissue and modulating the interplay between CB1, PPARγ and TRPV channel gene transcription.

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Acknowledgements

This work was partially supported by a grant of the University of Camerino, University Research Projects—Fondo di Ateneo per la Ricerca.

Author information

Author notes

  1. Paolo Cocci and Michele Moruzzi have contributed equally to this work.

Authors and Affiliations

  1. School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III Da Varano, 62032, Camerino, MC, Italy

    Paolo Cocci, Gilberto Mosconi, Consuelo Amantini, Daniele Tomassoni & Francesco Alessandro Palermo

  2. Department of Medicine, University of Leipzig, Leipzig, Germany

    Michele Moruzzi

  3. School of Pharmacy, University of Camerino, Camerino, Italy

    Ilenia Martinelli, Maria Vittoria Micioni Di Bonaventura, Carlo Cifani, Seyed Khosrow Tayebati, Silvia Damiano & Giulio Lupidi

  4. Department of Molecular Medicine, “Sapienza” University of Rome, Rome, Italy

    Federica Maggi

Authors
  1. Paolo Cocci
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  2. Michele Moruzzi
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  3. Ilenia Martinelli
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  4. Federica Maggi
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  5. Maria Vittoria Micioni Di Bonaventura
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  6. Carlo Cifani
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  7. Gilberto Mosconi
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  8. Seyed Khosrow Tayebati
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  9. Silvia Damiano
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  10. Giulio Lupidi
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  11. Consuelo Amantini
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  12. Daniele Tomassoni
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  13. Francesco Alessandro Palermo
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Corresponding author

Correspondence to Francesco Alessandro Palermo.

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Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical approval

All procedures were carried out in accordance with the Institutional Guidelines and complied with the Italian Ministry of Health (Prot. n. 1610/2013) and associated guidelines from European Communities Council Directive (EU Directive 2010/63/EU). The protocol was approved by the Ethics Committee of the University of Camerino (n. 7/2012, June 6, 2012).

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Cocci, P., Moruzzi, M., Martinelli, I. et al. Tart cherry (Prunus cerasus L.) dietary supplement modulates visceral adipose tissue CB1 mRNA levels along with other adipogenesis-related genes in rat models of diet-induced obesity. Eur J Nutr 60, 2695–2707 (2021). https://doi.org/10.1007/s00394-020-02459-y

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  • DOI: https://doi.org/10.1007/s00394-020-02459-y

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