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Diets rich in saturated fat and/or salt differentially modulate atrial natriuretic peptide and renin expression in C57BL/6 mice

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Abstract

Purpose

To study the effects of a diet rich in salt and/or saturated fat on atrial natriuretic peptide (ANP)-granules, hypertension, renin expression, and cardiac structure in C57Bl/6 mice.

Methods

Young adult male mice were separated into four groups (n = 12) and fed one of the following for 9 weeks: standard chow/normal salt (SC-NS), high-fat chow/normal salt (HF-NS), standard chow/high salt (SC-HS) and high-fat chow/high salt (HF-HS). Alterations in the serum ANP, ultrastructural analysis of cardiomyocytes that produce ANP, structural analysis of the left ventricle, blood pressure, renin expression, glomerular filtration rate (GFR), feed efficiency, and lipid and glucose parameters were examined.

Results

The HF-NS diet showed a small increase in ANP production and left ventricular hypertrophy, increased food efficiency, and abnormal lipid and glucose parameters. The SC-HS diet showed a large increase in ANP granules in myocytes and corresponding elevation in ANP serum levels, left ventricular hypertrophy, hypertension, decrease in renin levels, and increase in GFR. The combination of the two diets (HF-HS) had an additive effect.

Conclusion

The incorporation of a high-fat high-salt diet induced ultrastructural changes in cardiomyocytes, increased the production of ANP and increased its serum level, and reduced the amount of renin in the kidney.

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Acknowledgments

The authors thank Mss. Nemesis M. L. S. Monteiro and Mr. Eduardo M. Costa, for their help in the care of the animals. Furthermore, they thank Mrs. Thatiany Marinho and Angelica Figeuiredo for their technical assistance. This research was supported by grants from Brazilian agencies, CNPq (Conselho Nacional de Ciencia e Tecnologia, Ministerio da Ciencia e Tecnologia, Brazil) and FAPERJ (Fundaçao do Amparo a Pesquisa do Estado do Rio de Janeiro).

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Authors and Affiliations

  1. Laboratory of Morphometry, Metabolism and Cardiovascular, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Av 28 de Setembro 87 fds, Rio de Janeiro, RJ, 20551-030, Brazil

    Milton Vieira Costa, Caroline Fernandes-Santos, Tatiane da Silva Faria, Marcia Barbosa Aguila & Carlos Alberto Mandarim-de-Lacerda

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  1. Milton Vieira Costa
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  2. Caroline Fernandes-Santos
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  3. Tatiane da Silva Faria
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  4. Marcia Barbosa Aguila
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  5. Carlos Alberto Mandarim-de-Lacerda
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Correspondence to Carlos Alberto Mandarim-de-Lacerda.

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Costa, M.V., Fernandes-Santos, C., Faria, T.d.S. et al. Diets rich in saturated fat and/or salt differentially modulate atrial natriuretic peptide and renin expression in C57BL/6 mice. Eur J Nutr 51, 89–96 (2012). https://doi.org/10.1007/s00394-011-0196-1

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  • DOI: https://doi.org/10.1007/s00394-011-0196-1

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