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Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells

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Abstract

Background

Lycopene is a carotenoid whose biological activities and protective effect on prostate and breast cancer have been described, but little is known on its extra-intestinal metabolism and storage. While most alimentary lycopene is in all-trans configuration, in animal and human tissues approximately half of the lycopene is in cis isoforms.

Aim of study

Our object was to monitor the capacity of storage, isomerisation, and intracellular localization of all-trans and cis lycopene in hepatic stellate cells, which are the major sites of metabolism and storage of retinoids and carotenoids in the body.

Methods

We used the GRX cell line representative of murine hepatic stellate cells, incubated with 1–30 μM lycopene in culture medium. Analysis was done by high-performance liquid chromatography.

Results

Lycopene was able to induce expression of the lipocyte phenotype and it was internalized into GRX cells. Its cellular release only occurred in presence of albumin with a rapid initial decrease of intracellular lycopene. A corresponding increase in the culture medium was observed at 24 h. All-trans, 13-cis and 9-cis lycopene isoforms were identified in all the cell compartments. The membrane fraction contained the major part of lycopene, followed by the cytoplasmic fraction, lipid droplets and nuclei. The ratio between all-trans and cis isomers was approximately 2/1 in the majority parts of cell compartments.

Conclusions

This study identified a novel hepatic cell type able to store and isomerise lycopene. Liver can contribute to the serum and tissue equilibrium of cis/trans isomers of lycopene, and to participate in storage of lycopene under high extracellular concentration such as observed after the alimentary input.

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Acknowledgments

We thank Adriana Mercadante and Itaciara Nunes for help in spectrum-UV analyses of lycopene isomer standards. This study was sponsored by CNPq grants of the Brazilian Ministry of Science and Technology and by FAPERJ grants of the Rio de Janeiro State Government.

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Authors and Affiliations

  1. Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Anderson J. Teodoro, Daniel Perrone, Renata B. Martucci & Radovan Borojevic

  2. Laboratório de Bioquímica Nutricional e de Alimentos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Caixa Postal 68021, Rio de Janeiro, RJ, 21941-970, Brazil

    Anderson J. Teodoro, Daniel Perrone, Renata B. Martucci & Radovan Borojevic

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  1. Anderson J. Teodoro
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  2. Daniel Perrone
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  4. Radovan Borojevic
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Correspondence to Radovan Borojevic.

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Teodoro, A.J., Perrone, D., Martucci, R.B. et al. Lycopene isomerisation and storage in an in vitro model of murine hepatic stellate cells. Eur J Nutr 48, 261–268 (2009). https://doi.org/10.1007/s00394-009-0001-6

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  • DOI: https://doi.org/10.1007/s00394-009-0001-6

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