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Defatted milled grape seed protects adriamycin-treated hepatocytes against oxidative damage

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Abstract

Defatted milled grape seed (DMGS) is a wine by-product obtained from the oil extraction of the grape seed that contains different types of phenolic compounds. The present study was designed to evaluate the possible protective effect of DMGS on toxicity induced by adriamycin (ADR) in isolated rat hepatocytes. The study was carried out by examining the results of lactate dehydrogenase (LDH) release to estimate cytotoxicity; the thiobarbituric acid reactant substances (TBARS) and carbonyl group levels were measured as biomarkers of oxidative stress and ATP and GSH levels as estimation of intracellular effect. The results showed that DMGS extract protects the cellular membrane from oxidative damage and consequently prevents protein and lipid oxidation. The levels of ATP and GSH changes for the ADR toxicity were restored to control value in the presence of DMGS extract. The experimental results suggest that this wine by-product may be used to decrease oxidative stress.

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Abbreviations

DMGS:

defatted milled grape seed

LDH:

lactate dehydrogenase

TBARS:

thiobarbituric acid reactant substances

GSH:

glutathione

ADR:

adriamycin

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Acknowledgements

The authors want to grateful to the programs FEDER (F-1FD97-1471/QUI) for their financial support. We thank Miss Yolanda Fernandez for her help with the English revision.

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Authors and Affiliations

  1. Depto. de Pediatría, Ginecología y Obstetricia Facultad de Medicina, Universidad de Valencia, Valencia, Spain

    Victoria Valls-Belles, Mari Carmen Torres & Pilar Codoñer-Franch

  2. Depto. de Biotecnología y Ciencia de los Alimentos, Universidad de Burgos, Burgos, Spain

    Pilar Muñiz & Sagrario Beltran

  3. Área Bioquímica y Biología Molecular Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001, Burgos, Spain

    Pilar Muñiz

  4. Escuela de Enfermería, Universidad Complutense, Madrid, Spain

    Jesús Roman Martinez-Álvarez

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  1. Victoria Valls-Belles
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  2. Mari Carmen Torres
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  3. Pilar Muñiz
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Correspondence to Pilar Muñiz.

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Valls-Belles, V., Torres, M., Muñiz, P. et al. Defatted milled grape seed protects adriamycin-treated hepatocytes against oxidative damage. Eur J Nutr 45, 251–258 (2006). https://doi.org/10.1007/s00394-006-0591-1

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  • DOI: https://doi.org/10.1007/s00394-006-0591-1

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