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Association of ulcerative colitis with solute-linked carrier family 26 member A3 gene polymorphisms and its expression in colonic tissues in Chinese patients

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Abstract

Purpose

Abnormalities of the solute-linked carrier family 26 member A3 (SLC26A3) are implicated in the pathogenesis of several diseases including ulcerative colitis (UC). The short communication aimed at investigating the associations of UC with SLC26A3 (rs17154444, rs7810937, rs7785539, rs2108225 and rs6951457) polymorphisms and its expression in colonic tissues.

Methods

The techniques of SNaPshot method, quantitative real-time PCR and immunohistochemical analysis were conducted.

Results and conclusion

We found that the rs2108225 variation in SLC26A3 might increase the risk of UC and affect its expression at both the mRNA and protein levels in colonic tissues of patients with UC. Moreover, the rs17154444 variation might influence the severity of UC.

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References

  1. Xiao F, Juric M, Li J et al (2012) Loss of downregulated in adenoma (DRA) impairs mucosal HCO3(-) secretion in murine ileocolonic inflammation. Inflamm Bowel Dis 18(1):101–111. https://doi.org/10.1002/ibd.21744

    Article  PubMed  Google Scholar 

  2. Wojtal KA, Eloranta JJ, Hruz P (2009) Changes in mRNA expression levels of solute carrier transporters in inflammatory bowel disease patients. Drug Metab Dispos 37(9):1871–1877. https://doi.org/10.1124/dmd.109.027367

    Article  PubMed  CAS  Google Scholar 

  3. Ghishan FK, Kiela PR (2014) Epithelial transport in inflammatory bowel diseases. Inflamm Bowel Dis 20(6):1099–1109. https://doi.org/10.1097/MIB.0000000000000029.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Ishimori S, Kaito H, Matsunoshita N et al (2013) SLC26A3 gene analysis in patients with Bartter and Gitelman syndromes and the clinical characteristics of patients with unidentified mutations. Kobe J Med Sci 59(2):E36–E43. https://doi.org/10.1016/j.mam.2012.07.009

    Article  PubMed  CAS  Google Scholar 

  5. Hu D-y, Shao X-x, Xu C-l et al (2014) Associations of FUT2 and FUT3 gene polymorphisms with Crohn’s disease in Chinese patients. J Gastroenterol Hepatol 29(10):1778–1785. https://doi.org/10.1111/jgh.12599

    Article  PubMed  CAS  Google Scholar 

  6. Borenshtein D, Schlieper KA, Rickman BH et al (2009) Decreased expression of colonic Slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice. Infect Immun 77(9):3639–3650. https://doi.org/10.1128/IAI.00225-09

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. Asano K, Matsushita T, Umeno J et al (2009) A genome-wide association study identifies three new susceptibility loci for ulcerative colitis in the Japanese population. Nat Genet 41(12):1325–1329. https://doi.org/10.1038/ng.482

    Article  PubMed  CAS  Google Scholar 

  8. Asano K, Esaki M, Umeno J et al (2015) Contribution of susceptibility variants at FCGR2A and 13q12 to the risk of relapse among Japanese patients with ulcerative colitis. J Gastroenterol 50(11):1094–1102. https://doi.org/10.1007/s00535-015-1062-3

    Article  PubMed  CAS  Google Scholar 

  9. Yang SK, Jung Y, Kim H et al (2011) Association of FCGR2A, JAK2 or HNF4A variants with ulcerative colitis in Koreans. Dig Liver Dis 43(11):856–861. https://doi.org/10.1016/j.dld.2011.07.006

    Article  PubMed  CAS  Google Scholar 

  10. Liu JZ, van Sommeren S, Huang H et al (2015) Association analyses identify 38 susceptibility loci for inflammatory bowel disease and highlight shared genetic risk across populations. Nat Genet 47(9):979–986. https://doi.org/10.1038/ng.3359

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Cleynen I, Boucher G, Jostins L et al (2016) Inherited determinants of Crohn’s disease and ulcerativecolitis phenotypes: a genetic association study. Lancet 387:156–167. https://doi.org/10.1016/S0140-6736(15)00465-1

    Article  PubMed  PubMed Central  Google Scholar 

  12. Moyer RA, Wang D, Papp AC et al (2011) Intronic polymorphisms affecting alternative splicing of human dopamine D2 receptor are associated with cocaine abuse. Neuropsychopharmacology 36(4):753–762. https://doi.org/10.1038/npp.2010.208

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. Xiao-xiao Shao and Dao-po Lin contributed equally to this work.

Authors and Affiliations

  1. Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 109 West Xueyuan Road, Wenzhou, 325000, Zhejiang Province, China

    Xiao-xiao Shao, Dao-po Lin, Liang Sun, Chao-qun Wu, Wei Yang & Yi Jiang

Authors
  1. Xiao-xiao Shao
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  2. Dao-po Lin
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  3. Liang Sun
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  4. Chao-qun Wu
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  5. Wei Yang
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  6. Yi Jiang
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Contributions

Xiao-xiao Shao, Dao-po Lin, Liang Sun, Chao-qun Wu, Wei Yang and Yi Jiang all met all the criteria for the definition of authorship and contributed substantially to the manuscript.

Corresponding author

Correspondence to Yi Jiang.

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The authors declare that they have no competing interests.

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Shao, Xx., Lin, Dp., Sun, L. et al. Association of ulcerative colitis with solute-linked carrier family 26 member A3 gene polymorphisms and its expression in colonic tissues in Chinese patients. Int J Colorectal Dis 33, 1169–1172 (2018). https://doi.org/10.1007/s00384-018-3097-4

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  • DOI: https://doi.org/10.1007/s00384-018-3097-4

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