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Relationship between the expression levels of CD4+ T cells, IL-6, IL-8 and IL-33 in the liver of biliary atresia and postoperative cholangitis, operative age and early jaundice clearance

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Abstract

Objective

To investigate the expression levels of CD4+ T cells, IL-6, IL-8 and IL-33 in liver tissue of BA, and the relationship with postoperative cholangitis, operative age and early jaundice clearance.

Methods

45 cases of jaundice treated in the hospital from June 2018 to May 2020 were analyzed retrospectively. The expression and distribution of these factors were detected by HE staining and immunohistochemistry, the total bilirubin level and the incidence of cholangitis were recorded, and the relationship between liver inflammation level and the postoperative incidence of cholangitis, age of operation and early jaundice clearance were compared.

Results

Immunohistochemistry showed that the expression of CD4+ T cells, IL-6, IL-8 and IL-33 in the BA group were higher than those in the CBD group. ROC curve analysis showed the AUC of CD4+ T cells, IL-6 and IL-8 were 0.869, 0.886 and 0.838, respectively. The expression level of CD4+ T cells was negatively correlated with the decline rate of TBIL 3 months after operation, and the expressions of IL-8 and IL-33 were negatively correlated with the decline rate of TBIL 1 week after operation.

Conclusion

The high expression of CD4+ T cells, IL-6, IL-8 and IL-33 in the BA liver tissue may lead to cholangitis and can be used as a predictor of early jaundice clearance. The degree of liver inflammation infiltration had nothing to do with the age of operation and is not a risk factor for postoperative cholangitis.

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Abbreviations

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

AUC:

Area under the curve

BA:

Biliary atresia

CBD:

Congenital biliary dilatation

DCs:

Dendritic cells

DBIL:

Direct bilirubin

GGT:

Gamma-glutamyl transferase

HE:

Hematoxylin–eosin staining

IL-6:

Interleukin-6

IL-8:

Interleukin-8

IL-33:

Interleukin-33

MOD:

Mean optical density

PBS:

Phosphate-buffered saline

ROC:

Receiver operating characteristic

TBIL:

Total bilirubin

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Funding

This work was supported by the National Natural Science Foundation of China (no: 81760099). This work was supported by the Science and Technology Planning Project of Zunyi City, Guizhou Province of China (no.: [2019] 104).

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Authors and Affiliations

  1. Department of Pediatric Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, China

    Kaizhi Zhang, Yutong Chen, Zebing Zheng, Chengyan Tang, Daiwei Zhu, Xingrong Xia, Lu Huang, Qing Du, Yuanmei Liu & Zhu Jin

  2. Department of Pediatric Surgery, Guizhou Children’s Hospital, Zunyi, 563000, China

    Kaizhi Zhang, Yutong Chen, Zebing Zheng, Chengyan Tang, Daiwei Zhu, Xingrong Xia, Lu Huang, Qing Du, Yuanmei Liu & Zhu Jin

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  1. Kaizhi Zhang
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Contributions

KZ, YC, ZJ, ZZ, CT, DZ, XX, LH, QD and YL made substantial contributions to the conception and design, acquisition of data or analysis and interpretation of data. KZ, YC and ZJ drafted the article and revised it critically for important intellectual content. ZJ approved the final version to be published.

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Correspondence to Zhu Jin.

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The authors have indicated that they have no potential conflicts of interest to disclose.

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Affiliated Hospital of Zunyi Medical University approved the study protocol (Ethics Review Document KLLY-2019-133).

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Zhang, K., Chen, Y., Zheng, Z. et al. Relationship between the expression levels of CD4+ T cells, IL-6, IL-8 and IL-33 in the liver of biliary atresia and postoperative cholangitis, operative age and early jaundice clearance. Pediatr Surg Int 38, 1939–1947 (2022). https://doi.org/10.1007/s00383-022-05258-0

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