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Tracheal defect repair using a PLGA–collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel

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Abstract

Purpose

We studied the regenerated cartilage in tracheal defect repair and compared the bio-materials used versus native trachea using basic fibroblast growth factor (bFGF)-impregnated gelatin hydrogel.

Materials and methods

A full-thickness anterior defect was created in the cervical trachea of 15 experimental rabbits. The defect was implanted with a hybrid scaffold of poly(lactic-co-glycolic acid) (PLGA) knitted mesh and collagen sponge. The implanted trachea was reinforced with a copolymer stent of polycaprolactone and poly(lactic acid) coarse fiber mesh. A gelatin hydrogel was used for providing a sustained release of bFGF. The reconstructed tracheas were divided into three groups with wrapped materials; without gelatin hydrogel (control group, n = 5), a gelatin hydrogel with saline (gelatin group, n = 5), and a gelatin hydrogel with 100 μg of bFGF (bFGF group, n = 5). One of the five rabbits in each group at 1 month after operation, one at 3 months, and three at 6 months were killed and the engineered tracheas were evaluated histologically. Biomechanical properties were evaluated on samples at 6 months postoperatively.

Results

The rigid support in the defect portion was maintained during 6 months postoperatively. The newly regenerated cartilages were recognized between the host cartilage stumps at 3 months postoperatively in the bFGF group, and limited new cartilage growth and epithelialization were observed at 6 months postoperatively.

Conclusions

The experiment shows that using bFGF, better mechanical strength was obtained but with poor cartilage growth.

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Acknowledgments

The authors thank Kouki Hagiwara for their expert technical assistance in these experiments. This work was supported by a Grant-in-Aid for Exploratory Research and a Grant-in-Aid for Scientific Research (KAKENHI) from the Japan Society for the Promotion of Science (19659457).

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Authors and Affiliations

  1. Department of Pediatric Surgery, Graduate School of Comprehensive Human Sciences and University Hospital, University of Tsukuba, Tsukuba, 305-8575, Japan

    Yukihiro Tatekawa, Hiroaki Komuro & Michio Kaneko

  2. Biomaterials Center, National Institute for Materials Science, Tsukuba, Japan

    Naoki Kawazoe & Guoping Chen

  3. Artificial Organ and Biomaterials Group, Tsukuba, Japan

    Yoshio Shirasaki

Authors
  1. Yukihiro Tatekawa
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  2. Naoki Kawazoe
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  3. Guoping Chen
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  4. Yoshio Shirasaki
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  5. Hiroaki Komuro
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  6. Michio Kaneko
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Corresponding author

Correspondence to Yukihiro Tatekawa.

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Tatekawa, Y., Kawazoe, N., Chen, G. et al. Tracheal defect repair using a PLGA–collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel. Pediatr Surg Int 26, 575–580 (2010). https://doi.org/10.1007/s00383-010-2609-2

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  • DOI: https://doi.org/10.1007/s00383-010-2609-2

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