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Oral insulin stimulates intestinal epithelial cell turnover in correlation with insulin-receptor expression along the villus–crypt axis in a rat model of short bowel syndrome

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Abstract

Purpose

It has been reported that oral insulin (OI) has a trophic effect on intestinal mucosa. In the present study, we evaluated the effect of OI on enterocyte turnover and correlated it with insulin-receptor expression along the villus–crypt axis in a rat model of short bowel syndrome (SBS).

Methods

Male rats were divided into three groups: Sham rats underwent bowel transection, SBS rats underwent a 75% bowel resection, and SBS–OI rats underwent bowel resection and were treated with OI given in drinking water (1 U/ml) from the fourth postoperative day. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined on day 15. Real-time PCR was used to determine the level of insulin receptor-beta (IRB) mRNA. Insulin-receptor expression along the villus–crypt axis (villus tips, lateral villi and crypts) was assessed by immunohistochemistry. The effect of OI on cell turnover for each compartment was evaluated in correlation with the receptor expression. Statistical analysis was performed using the one-way ANOVA test, with P < 0.05 considered statistically significant.

Results

Treatment with OI resulted in a significant increase in all parameters of intestinal adaptation. Insulin-receptor expression in crypts significantly increased in SBS rats (vs. Sham rats) and was accompanied by a significant increase in enterocyte proliferation following OI administration. A significant increase in insulin-receptor expression at the tip of the villous and in the lateral villous in SBS rats (vs. Sham) was accompanied by decreased cell apoptosis in these compartments following treatment with OI.

Conclusions

In a rat model of SBS, OI enhances enterocyte turnover and stimulates intestinal adaptation. The stimulating effect of insulin on enterocyte turnover correlates with insulin-receptor expression along the villus–crypt axis.

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Acknowledgments

This work was supported by an Israel Science Foundation (ISF) research grant.

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Authors and Affiliations

  1. Laboratory of Intestinal Adaptation and Recovery, Technion-Israel Institute of Technology, The Ruth & Bruce Rappaport Faculty of Medicine, Haifa, Israel

    Shani Ben Lulu

  2. Department of Pediatric Surgery B, Bnai Zion Medical Center, 47 Golomb St., P.O.B. 4940, 31048, Haifa, Israel

    Jorge G. Mogilner & Igor Sukhotnik

  3. Department of Pediatric Gastroenterology and Nutrition, Meyer Children’s Hospital, Rambam Medical Center, Haifa, Israel

    Ron Shaoul

  4. Department of Pediatrics, Meyer Children’s Hospital, Rambam Medical Center, Haifa, Israel

    Naim Shehadeh

  5. Institute of Gastroenterology, Nutrition and Liver Diseases, Schneider Children’s Medical Center, Sackler School of Medicine, Tel-Aviv University, Tel Aviv, Israel

    Raanan Shamir

  6. Section of Pediatric Surgery, C.S. Mott Children’s Hospital, University of Michigan Medical School, Ann Arbor, MI, USA

    Arnold G. Coran

Authors
  1. Shani Ben Lulu
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  2. Arnold G. Coran
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  3. Jorge G. Mogilner
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  4. Ron Shaoul
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  5. Raanan Shamir
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  6. Naim Shehadeh
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  7. Igor Sukhotnik
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Corresponding author

Correspondence to Igor Sukhotnik.

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Ben Lulu, S., Coran, A.G., Mogilner, J.G. et al. Oral insulin stimulates intestinal epithelial cell turnover in correlation with insulin-receptor expression along the villus–crypt axis in a rat model of short bowel syndrome. Pediatr Surg Int 26, 37–44 (2010). https://doi.org/10.1007/s00383-009-2520-x

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  • DOI: https://doi.org/10.1007/s00383-009-2520-x

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