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Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis

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Abstract

Introduction

MicroRNAs (miRNAs) are small (∽22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.

Methods

This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.

Results

A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513–1, hsa-miR-513–2, hsa-miR-518c*, hsa-miR-129–1, hsa-miR-129–2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.

Conclusion

These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.

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Acknowledgements

We thank the patients, the normal individuals, and their family members for taking part in this study. This work was supported in part by the National Basic Research Program of China (2007CB511905), the National Infrastructure Program of Chinese Genetic Resources (2006DKA21301), National Natural science Foundation of China (30872843), the Program for Innovative Researcher Team in Science and Technology in Fujian Province University (FMU-RT001) and Fujian Provincial Natural Science Foundation (C0520003, C0810018).

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Authors and Affiliations

  1. Peking Union Medical College, Tsinghua University, Beijing, China

    Jian-Jun Zhao, Jianhong Lin, Nan Yao & Xu Ma

  2. Department of Genetics, National Research Institute for Family Planning, Peking Union Medical College, Tsinghua University, Beijing, China, 100081

    Jian-Jun Zhao, Juhua Yang, Nan Yao & Xu Ma

  3. Biomedical Engineering Center, Fujian Medical University, Fuzhou, Fujian, China

    Juhua Yang & Jianhua Xu

  4. Department of Ophthalmology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China

    Yihua Zhu & Jianlong Zheng

  5. Key Laboratory of Infection and Oncology, Molecular Medicine Research Center of Fujian Medical University, Fujian Province University, Fuzhou, Fujian, China, 350004

    Jian-Yin Lin

  6. H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, FL, USA

    Jian-Jun Zhao, Jianhong Lin & Jin Q. Cheng

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  1. Jian-Jun Zhao
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  2. Juhua Yang
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  4. Nan Yao
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  9. Jian-Yin Lin
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Corresponding authors

Correspondence to Jian-Yin Lin or Xu Ma.

Additional information

Jian-Jun Zhao and Juhua Yang contribute equally to the article.

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Zhao, JJ., Yang, J., Lin, J. et al. Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis. Childs Nerv Syst 25, 13–20 (2009). https://doi.org/10.1007/s00381-008-0701-x

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  • DOI: https://doi.org/10.1007/s00381-008-0701-x

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