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Low prevalence of HPV detection and genotyping in non-muscle invasive bladder cancer using single-step PCR followed by reverse line blot

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Abstract

Purpose

To clarify the role of human papillomavirus (HPV) in non-muscle invasive bladder cancer, HPV-DNA was scrutinized in formalin-fixed, paraffin-embedded (FFPE) bladder cancer tissue using single-step PCR (HPV L1) for HPV detection, followed by reverse line blot (RLB) for genotyping.

Methods

A total of 186 patients who underwent transurethral resection of the bladder due to primary, non-muscle invasive bladder cancer from 2006 to 2009 were reviewed. A positive control group of 22 cervical tissues with cervical carcinoma was included.

Results

Histology confirmed urothelial carcinoma in all patients: primary CIS, pTa, pT1 and pTa + pT1 in 14 (7.5 %), 134 (72 %), 36 (19.4 %) and two (1.1 %) patients, respectively. A total of 119 (63.9 %) of them were classified as low-risk, while 67 (36.1 %) were high-risk cancers. Tumor recurrence and progression (≥pT2) were seen in 79 and 11 patients (mean follow-up 45 months). The presence of HPV-DNA by single-step PCR was detected in four (2.2 %) patients. HPV 16 and HPV 6 were positive in two (1.1 %) and one (0.6 %) patient, respectively In one case, no HPV genotype listed on the RLB assay could be identified. In the control group, the HPV infection rate was 100 %: HPV 16 in 12 (54.6 %) patients, HPV 16/18 in four (18.3 %) patients, HPV 18 in two (9.1 %) patients, HPV 16/45 in one patient (4.5 %), HPV 18/33 in one (4.5 %) patient, HPV 16/33 in one (4.5 %) patient and HPV 33 in one (4.5 %) patient.

Conclusions

Our study demonstrates low prevalence of HPV infection in FFPE bladder cancer tissue, arguing against the etiological role of HPV in non-muscle urothelial carcinogenesis.

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Acknowledgments

The authors want to thank Julia Heppke for excellent technical assistance. This research was supported by a grant from the Medical Research Foundation Tyrol (MFF Tyrol, Project Number 255). The funders had no role in study design, data collection and analysis, or decision to publish.

Conflict of interest

The authors had no conflicts of interest to declare.

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Authors and Affiliations

  1. Division of Experimental Urology, Department of Urology, Medical University of Innsbruck, Innsbruck, Austria

    Renate Pichler, Zoran Culig, Sebastian List, Isabel Heidegger, Helmut Klocker, Wolfgang Horninger & Hannes Steiner

  2. Section of Virology, Department of Hygiene, Microbiology and Social Medicine, Innsbruck, Austria

    Wegene Borena & Dorothee Von Laer

  3. Division of General Pathology, Department of Pathology, Medical University Innsbruck, Innsbruck, Austria

    Georg Schäfer, Claudia Manzl & Andrea Brunner

  4. Department of Medical Statistics, Informatics and Health Economics, Müllerstrasse 44A-6020, Innsbruck, Austria

    Sabrina Neururer

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  1. Renate Pichler
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Correspondence to Andrea Brunner.

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Pichler, R., Borena, W., Schäfer, G. et al. Low prevalence of HPV detection and genotyping in non-muscle invasive bladder cancer using single-step PCR followed by reverse line blot. World J Urol 33, 2145–2151 (2015). https://doi.org/10.1007/s00345-015-1539-y

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  • DOI: https://doi.org/10.1007/s00345-015-1539-y

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