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Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage

  • Aquaculture and Fisheries
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Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus (WSSV) in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei (LvPT) was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigen™ design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at ~27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA (4 μg per shrimp) was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection (hpi). Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.

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Acknowledgment

We sincerely thank the Guangtai Company (Hainan, China) for providing the postlarval shrimp for this study.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Shijun Xie  (谢世筠), Fuhua Li  (李富花), Xiaojun Zhang  (张晓军), Jiquan Zhang  (张继泉) & Jianhai Xiang  (相建海)

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Shijun Xie  (谢世筠)

  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Fuhua Li  (李富花) & Jianhai Xiang  (相建海)

Authors
  1. Shijun Xie  (谢世筠)
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  2. Fuhua Li  (李富花)
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  3. Xiaojun Zhang  (张晓军)
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  4. Jiquan Zhang  (张继泉)
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  5. Jianhai Xiang  (相建海)
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Corresponding author

Correspondence to Jianhai Xiang  (相建海).

Additional information

Supported by the National Basic Research Program of China (973 Program) (No. 2012CB114403) and the China Agriculture Research System-47 (CARS-47)

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Xie, S., Li, F., Zhang, X. et al. Peritrophin-like protein from Litopenaeus vannamei (LvPT) involved in white spot syndrome virus (WSSV) infection in digestive tract challenged with reverse gavage. Chin. J. Ocean. Limnol. 35, 1524–1530 (2017). https://doi.org/10.1007/s00343-017-6109-2

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  • DOI: https://doi.org/10.1007/s00343-017-6109-2

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