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Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino

  • Aquaculture and Fisheries
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Abstract

Temperature and salinity are two of the most potent abiotic factors influencing marine mollusks. In this study, we investigated the individual and combined effects of temperature and salinity on the survival and growth of juvenile Pacific abalone, Haliotis discus hannai Ino, and also examined the DNA methylation alteration that may underpin the phenotypic variation of abalone exposed to different rearing conditions. The single-factor data showed that the suitable ranges of temperature and salinity were 16–28°C at a constant salinity of 32, and 24–40 at a constant temperature of 20°C, respectively. The two-factor data indicated that both survival and growth were significantly affected by temperature, salinity and their interaction. The optimal temperature-salinity combination for juveniles was 23–25°C and 30–36. To explore environment-induced DNA methylation alteration, the methylation-sensitive amplified polymorphism (MSAP) technique was used to analyze the genomic methylation profiles of abalone reared in optimal and adverse conditions. Neither temperature nor salinity induced evident changes in the global methylation level, but 67 and 63 differentially methylated loci were identified in temperature and salinity treatments, respectively. The between-group eigen analysis also showed that both temperature and salinity could induce epigenetic differentiation in H. discus hannai Ino. The results of our study provide optimal rearing conditions for juvenile H. discus hannai Ino, and represent the first step toward revealing the epigenetic regulatory mechanism of abalone in response to thermal and salt stresses.

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Acknowledgments

We wish to thank Dr. Q. JIANG and Dr. C. CHEN for providing support and help in the experimental process.

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Ning Kong  (孔宁), Xiao Liu  (刘晓) & Junyuan Li  (李俊元)

  2. Ningbo University, Ningbo, 315211, China

    Wendan Mu  (穆文丹), Jianwu Lian  (连建武) & Yanjie Xue  (薛艳洁)

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Ning Kong  (孔宁) & Junyuan Li  (李俊元)

  4. Ocean University of China, Qingdao, 266003, China

    Qi Li  (李琪)

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  1. Ning Kong  (孔宁)
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  2. Xiao Liu  (刘晓)
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  3. Junyuan Li  (李俊元)
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  4. Wendan Mu  (穆文丹)
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  5. Jianwu Lian  (连建武)
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  6. Yanjie Xue  (薛艳洁)
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  7. Qi Li  (李琪)
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Correspondence to Qi Li  (李琪).

Additional information

Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A412)

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Kong, N., Liu, X., Li, J. et al. Effects of temperature and salinity on survival, growth and DNA methylation of juvenile Pacific abalone, Haliotis discus hannai Ino. Chin. J. Ocean. Limnol. 35, 1248–1258 (2017). https://doi.org/10.1007/s00343-016-5185-z

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  • DOI: https://doi.org/10.1007/s00343-016-5185-z

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