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Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus

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Abstract

To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1°C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1°C. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly (P < 0.05) as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1°C and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8°C, and creatinine concentration was highest at 3°C. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly (P < 0.05) as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5°C may induce a stress response in S. maximus and that temperature should be kept above 8°C in the aquaculture setting to avoid damage to the fish.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences, Qingdao, 266071, China

    Liqin Ji  (纪利芹), Keyong Jiang  (蒋克勇), Mei Liu  (刘梅), Baojie Wang  (王宝杰), Longjiang Han  (韩龙江), Mingming Zhang  (张明明) & Lei Wang  (王雷)

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Liqin Ji  (纪利芹)

  3. Fisheries College, Ocean University of China, Qingdao, 266003, China

    Longjiang Han  (韩龙江) & Mingming Zhang  (张明明)

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  1. Liqin Ji  (纪利芹)
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  2. Keyong Jiang  (蒋克勇)
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  3. Mei Liu  (刘梅)
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  7. Lei Wang  (王雷)
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Correspondence to Keyong Jiang  (蒋克勇) or Lei Wang  (王雷).

Additional information

Supported by the Special Foundation for Youth Science and Technology of Qingdao (No. 14-2-4-59-jch), the Science and Technology Development Planning of Shandong Province (No. 2011GGC21007), and the National Natural Science Foundation of China (No. 41206144).

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Ji, L., Jiang, K., Liu, M. et al. Low temperature stress on the hematological parameters and HSP gene expression in the turbot Scophthalmus maximus . Chin. J. Ocean. Limnol. 34, 430–440 (2016). https://doi.org/10.1007/s00343-016-4367-z

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  • DOI: https://doi.org/10.1007/s00343-016-4367-z

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