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Glass lab-on-a-chip platform fabricated by picosecond laser for testing tumor cells exposed to X-ray radiation

  • S.I. : COLA 2021/2022
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Abstract

Lab-on-a-chip strategies using miniaturized devices enable cells to be cultured in a tridimensional (3D) space that offers a real model mimicking in vivo environment. One may provide architectural configurations relevant for specific tissues to maintain them at adequate temperature, oxygen levels, and pH during the necessary time intervals for observation. Herein, we propose a miniaturized lab-on-chip glass device suitable for simultaneous dosimetry measurements and evaluation of the biological effects of ionizing radiation on cancer cells. For the 3D fabrication of biologically relevant microenvironment, high repetition rate picosecond laser-assisted etching is applied to create microfluidic networks between sealed cell culture chambers in photo-sensitive glasses (PG). To evaluate the radiation dose, we employed collimated X-ray beams to generate free electrons in the PG samples by photoreduction of Ag ions to Ag atoms. A subsequent thermal treatment applied to the PG induced clustering of precipitated Ag atoms to color the exposed area to brown, which allows us to directly evaluate a threshold of the applied X-ray radiation dose applied directly on chip. Based on our glass biochip, we tested the response of human melanoma cancer cells exposed to various X-ray doses. This lab-on-chip platform is a valuable tool to analyze and validate the cellular response to new irradiation strategies as alternatives to conventional radiotherapy methods.

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Acknowledgements

This research was supported by IFA (Institute of Atomic Physics) through ELI-RO_2020_11 Project, No. 01/2020 and Romanian Ministry of Education and Research, under Romanian National Nucleus Program LAPLAS VI under Contract No. 16N/2019. This work has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 871124 Laserlab-Europe.

Funding

The authors have no relevant financial or non-financial interests to disclose. This research received funding from IFA (Institute of Atomic Physics) through ELI-RO_2020_11 Project, No. 01/2020. This work has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 871124 Laserlab-Europe.

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Author notes

  1. C. E. Staicu and F. Jipa have contributed equally to this work.

Authors and Affiliations

  1. National Institute for Laser, Plasma and Radiation Physics, Bucharest, Romania

    C. E. Staicu, F. Jipa, I. Porosnicu, A. Bran, E. Stancu, C. Dobrea, E. Axente, I. Tiseanu & F. Sima

  2. Department of Anatomy, Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, 050095, Bucharest, Romania

    C. E. Staicu & B. M. Radu

  3. RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    F. Sima & K. Sugioka

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  1. C. E. Staicu
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Correspondence to F. Sima.

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Staicu, C.E., Jipa, F., Porosnicu, I. et al. Glass lab-on-a-chip platform fabricated by picosecond laser for testing tumor cells exposed to X-ray radiation. Appl. Phys. A 128, 770 (2022). https://doi.org/10.1007/s00339-022-05915-0

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