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Localization of Eutr2, a locus controlling susceptibility to DES-induced uterine inflammation and pyometritis, to RNO5 using a congenic rat strain

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Abstract

In some rat strains chronic administration of exogenous estrogens induces pyometritis, an inflammation of the uterus associated with infection, suggesting that there is genetic variation in susceptibility to estrogen-induced inflammation and pyometritis. In this article we report that following 10 weeks of treatment with the synthetic estrogen diethylstilbestrol (DES), Fisher 344 (F344) rats exhibit modest uterine inflammation and a 0% incidence of pyometritis. By contrast, under identical experimental conditions, Brown Norway (BN) rats exhibit significant inflammation and a 100% incidence of pyometritis. Similarly, we also observed profound uterine inflammation and a 100% incidence of pyometritis in a congenic rat strain in which a segment of RNO5 from the BN strain is carried on the F344 strain. These data suggest that a locus on RNO5 controls both the magnitude of DES-induced uterine inflammation and susceptibility to DES-induced pyometritis. This locus, designated Eutr2, resides within the same segment of RNO5 as the Eutr1 locus, which confers susceptibility to E2-induced pyometritis in an F2 population generated in a cross between the BN and August × Copenhagen 9935, Irish (ACI) strains.

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Acknowledgments

The authors gratefully acknowledge Phillip Doyle for technical assistance. Financial support was provided by Oakland University Research in Excellence Program in Biotechnology and by the National Institutes of Health grant R01-CA71911.

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Authors and Affiliations

  1. Department of Biological Sciences, Oakland University, Rochester, Michigan, 48309, USA

    Jyotsna Pandey & Douglas L. Wendell

  2. Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA

    Karen A. Gould & James D. Shull

  3. Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA

    Karen A. Gould & James D. Shull

  4. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198, USA

    Rodney D. McComb & James D. Shull

Authors
  1. Jyotsna Pandey
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  2. Karen A. Gould
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  3. Rodney D. McComb
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  4. James D. Shull
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  5. Douglas L. Wendell
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Corresponding author

Correspondence to Karen A. Gould.

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Jyotsna Pandey, Karen A. Gould contributed equally to this work.

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Pandey, J., Gould, K.A., McComb, R.D. et al. Localization of Eutr2, a locus controlling susceptibility to DES-induced uterine inflammation and pyometritis, to RNO5 using a congenic rat strain. Mamm Genome 16, 865–872 (2005). https://doi.org/10.1007/s00335-005-0071-6

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  • DOI: https://doi.org/10.1007/s00335-005-0071-6

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