Abstract
Tigger elements are human DNA transposons homologous to the pogo element of Drosophila melanogaster. They contain an open reading frame for a transposase very similar to the major mammalian centromere protein CENP-B. We found in the horse genome a DNA element (Ecatig3) sharing 88% homology with human Tigger3. The presence of Tigger elements in the horse genome confirms previous data that date these elements before the divergence between Perissodactyla and Primates (80–90 Myr ago). Copy number evaluation indicates that the horse element is much more abundant than its human counterpart. Southern blot analysis demonstrates that Ecatig3 elements are extremely homogeneous; this may indicate that the evolution of this DNA transposon has been driven by some kind of selection and has not been neutral.
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Acknowledgments
We thank Prof. A.R. Malacrida, Department of Animal Biology, University of Pavia, for helpful suggestions and discussion. This work was supported by grants from MURST to E. Raimondi (Cofinanziamento 2002–2003), and from the University of Pavia to L. De Carli (Progetto d’Ateneo) and to E. Raimondi (Fondo di Ateneo per la Ricerca).
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The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession number AY382599.
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Paulis, M., Moralli, D., Bensi, M. et al. Isolation from the horse genome of a new DNA transposonbelonging to the Tigger family. Mamm Genome 15, 399–403 (2004). https://doi.org/10.1007/s00335-004-3040-6
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DOI: https://doi.org/10.1007/s00335-004-3040-6