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Transgenic oat plants via visual selection of cells expressing green fluorescent protein

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Abstract

 New selectable markers and selection systems are needed to increase the efficiency and flexibility of plant transformation. The objective of this research was to determine if the green fluorescent protein (gfp) gene could be utilized as a visual selectable marker for transformation of oat (Avena sativa L.). A modified gfp gene was delivered into oat cells by microprojectile bombardment. Cell clusters expressing gfp were visually identified using fluorescence microscopy and physically isolated at each subculture. Eleven independent transgenic cell lines were obtained, and fertile plants regenerated from all lines. Transgene integration and expression were confirmed in transgenic plants and progeny. Transgene expression segregated in a 3 : 1 ratio in progeny of the majority of the transgenic lines.

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Authors and Affiliations

  1. Department of Agronomy, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA e-mail: hfkaeppl@facstaff.wisc.edu Fax: +1-608-2625217, , , , , , US

    H. F. Kaeppler & A. R. Carlson

  2. Novartis Seeds, Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA, , , , , , WF

    G. K. Menon

  3. USDA-ARS Cereal Crops Research Unit, 501N. Walnut Street, Madison, WI 53705, USA, , , , , , US

    R. W. Skadsen

  4. VTT Biotechnology and Food Research, P.O. Box 1505, FIN-02044 VTT, Finland, , , , , , FI

    A. M. Nuutila

Authors
  1. H. F. Kaeppler
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  2. G. K. Menon
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  3. R. W. Skadsen
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  4. A. M. Nuutila
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  5. A. R. Carlson
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Additional information

Received: 11 May 1999 / Revision received: 31 August 1999 / Accepted: 2 September 1999

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Kaeppler, H., Menon, G., Skadsen, R. et al. Transgenic oat plants via visual selection of cells expressing green fluorescent protein. Plant Cell Reports 19, 661–666 (2000). https://doi.org/10.1007/s002999900167

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  • DOI: https://doi.org/10.1007/s002999900167

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