Abstract
The metabolic relationship between littorine and hyoscyamine has been monitored in transformed roots of Datura stramonium. Quantification by GC of unlabelled littorine and by GCMS of 13C-labelled littorine demonstrated that exogenously added littorine (0.1 mm) was significantly metabolised (35%) to hyoscyamine. In contrast, exogenously added hyoscyamine was not metabolised to littorine, indicating that this conversion is irreversible. The conversion of littorine to hyoscyamine was suppressed by P-450 oxidase inhibitors (particularly clotrimazole), implicating the involvement, at least in part of a cytochrome P-450 activity operating hyoscyamine biosynthesis.
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Received: 15 September 1997 / Revision received: 14 February 1998 / Accepted: 10 March 1998
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Zabetakis, I., Edwards, R., Hamilton, J. et al. The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium. Plant Cell Reports 18, 341–345 (1998). https://doi.org/10.1007/s002990050583
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DOI: https://doi.org/10.1007/s002990050583