An improved optical technique for monitoring plant cell concentration

Abstract

 An improved optical method was developed to determine cell weight concentrations using a micro-plate reader. Light absorbance was measured by a vertical light beam, which can minimize the cell settling effect found in traditional optical measurements with a horizontal light beam. The use of well plates not only requires very small sample sizes, but also handles a large number of samples at the same time. Absorbance measurements were linearly related to cell weight over the full range of batch culture growth.