Abstract
Key message
Knock out OsABA8ox helps improve pre-harvest spouting resistance and do not affect rice yield. Pre-harvest sprouting(PHS) is a phenomenon that the seeds of crops germinate preharvest, which reduces the yield and quality of rice. Abscisic acid(ABA) is one of the phytohormones that promotes seed dormancy. ABA8’ hydroxylase is the main enzyme that can catabolism ABA in plant. There are three genes that encode ABA8’ hydroxylase in rice, named OsABA8ox1, OsABA8ox2 and OsABA8ox3. In this study, we use CRISPR/Cas9 gene editing technology to target these three genes in Ningjing6 and find that the knockout transgenic lines are all significantly strengthen in seed dormancy and have no effect on the yield. By a series of quantitative experiments, we consider that after knock out OsABA8ox, the high endogenous ABA level will influence the ABA signal which suppress the substantial and energy metabolism in the seeds, and finally led to higher dormancy.
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Acknowledgements
I would like to acknowledge others within Wan lab who helped give advice and direction on this project, such as Prof. Shirong Zhou and Mr. Song Cui.
Funding
This work was found by grants from National Transformation Science and Technology Program (2016ZX08001006), National Natural Science Foundation of China (31871712), National Key R&D Program of China (2021YFD1200504) and National Science Foundation of Jiangsu Province (BK20212010). The work was also supported by Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture, P. R. China, Jiangsu Collaborative Innovation Center for Modern Crop Production, and Southern Japonica Rice Research and Development Co. LTD.
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All authors contribute to the study conception and design. LJ and JM provided the idea and designed the experiments. KF, WS, CC, CM, YH, FZ, QH, PW, TM, YC, ZZ, MZ and QT performed the experiments. KF analyzed the data and wrote the manuscript. XL was responsible field management. All authors read and approved the final manuscript.
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Communicated by Neal Stewart.
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299_2022_2917_MOESM1_ESM.tif
Supplementary Fig. 1 (A) Germination percentages of seeds from Ningjing6 and T3 cr-aba8ox lines after storage for 18 months and (B) after storage for 12 months. (C) Expression levels of the genes that displayed the most significant DEGs. (D) Agronomic traits (tiller number, panicle length, primary branch number per panicle, grain length, grain width, 1000-grain weight, grain number per panicle, seed setting rate, and yield per plant) of Ningjing6 and cr-aba8ox lines. (E) Plant height of Ningjing4, Ningjing8 and their cr-aba8ox1 knock out mutant derivatives (TIF 403506 KB)
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Fu, K., Song, W., Chen, C. et al. Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9. Plant Cell Rep 41, 2107–2110 (2022). https://doi.org/10.1007/s00299-022-02917-3
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DOI: https://doi.org/10.1007/s00299-022-02917-3