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NAP is involved in GA-mediated chlorophyll degradation and leaf senescence by interacting with DELLAs in Arabidopsis

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Abstract

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RGA/GAI and NAP interacted with each other, and NAP was involved in GA signaling as a role of regulating age-dependent and dark-induced leaf senescence in Arabidopsis.

Abstract

Leaf senescence is a significant biological process which is beneficial for plant growth, development, and generation alternation in Arabidopsis. Recent researches have shown gibberellins (GAs) could accelerate leaf senescence. Nevertheless, the GA signaling involved in leaf senescence process remains elusive. Here, we reported a new potential regulation mechanism of GA-mediated chlorophyll degradation and leaf senescence. In this study, we confirmed that NAP positively regulated age-dependent and dark-induced leaf senescence and NAP knockout mutant nap was hyposensitive to GA3 (an active form of GA) treatment. DELLA family proteins with highly conserved structural domain function as master growth repressors that integrated GA signaling and leaf senescence. We validated RGA and GAI could interact with NAP in vitro and in vivo, and subsequently impaired the transcriptional activities of NAP to induce SAG113 and AAO3 expression in nap protoplasts. Taken together, we suggest that NAP is a novel component of the regulatory network that modulates the progress of leaf senescence in GA signaling.

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Abbreviations

NAP-OX :

NAP overexpression

BiFC:

Bimolecular fluorescence complementation

fLUC:

Firefly luciferase

GA:

Gibberellin

GAI :

GA-insensitive

RGA :

Repressor of ga1-3

RGL1 :

RGA-like 1

RGL2 :

RGA-like 2

RGL3 :

RGA-like 3

NYC1 :

Non-yellow coloring 1

PAC:

Paclobutrazol

qRT-PCR:

Real-time quantitative reverse

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Funding

This work was supported by the National Basic Research Program of China (973 Program) (2015CB150100), the National Natural Science Foundation of China (31670235 and 31970263 to HL; 31570237 to DZ), the National Research and Development Project of Transgenic Crops of China (2016ZX08009-003-002), Sichuan Natural Science Foundation (2019YFS0457) and Forage Innovation Team Supporting Fund from Sichuan Provincial Department of Agriculture, the Fundamental Research Funds for the Central Universities (SCU2019D013, SCU2018D006)

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Authors and Affiliations

  1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, 610064, China

    Wei Lei, Yan Li, Xiuhong Yao, Kang Qiao, Lin Wei, Dawei Zhang & Honghui Lin

  2. School of Life Science, Guangzhou University, Guangzhou, 510006, China

    Baohui Liu

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  1. Wei Lei
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  2. Yan Li
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  3. Xiuhong Yao
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  4. Kang Qiao
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  5. Lin Wei
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  6. Baohui Liu
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  7. Dawei Zhang
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  8. Honghui Lin
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Correspondence to Dawei Zhang or Honghui Lin.

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Communicated by Inhwan Hwang.

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Lei, W., Li, Y., Yao, X. et al. NAP is involved in GA-mediated chlorophyll degradation and leaf senescence by interacting with DELLAs in Arabidopsis. Plant Cell Rep 39, 75–87 (2020). https://doi.org/10.1007/s00299-019-02474-2

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