References
Fujiuchi N, Matoba N, Matsuda R (2016a) Environment control to improve recombinant protein yields in plants based on Agrobacterium-mediated transient gene expression. Front Bioeng Biotechnol 4:23
Fujiuchi N, Matsuda R, Matoba N, Fujiwara K (2016b) Removal of bacterial suspension water occupying the intercellular space of detached leaves after agroinfiltration improves the yield of recombinant hemagglutinin in a Nicotiana benthamiana transient gene expression system. Biotechnol Bioeng 113:901–906
Holtz BR, Berquist BR, Bennett LD, Kommineni VJM, Munigunti RK, White EL, Wilkerson DC, Wong KYI, Ly LH, Marcel S (2015) Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals. Plant Biotechnol J 13:1180–1190
Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol 23:718–723
Matsuda R, Tahara A, Matoba N, Fujiwara K (2012) Virus vector-mediated rapid protein production in Nicotiana benthamiana: effects of temperature and photosynthetic photon flux density on hemagglutinin accumulation. Environ Control Biol 50:375–381
Norikane JH (2015) The potential of LEDs in plant-based bio-pharmaceutical production. HortScience 50:1289–1292
Acknowledgements
We thank Shoko Miyagi and Haruko Maeyama for their technical assistance and Drs. Naomichi Fujiuchi and Nobuyuki Matoba for providing invaluable suggestions. The magnICON vector was provided by Icon Genetics GmbH. The pNM216 and N. benthamiana seeds were provided by Kentucky BioProcessing, Inc. This work was financially supported in part by Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry (Grant Number 25025A) and by JSPS KAKENHI (Grant Number JP26712021) to RM.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Communicated by Neal Stewart.
Rights and permissions
About this article
Cite this article
Matsuda, R., Abe, T. & Fujiwara, K. Viral vector-based transient gene expression in Nicotiana benthamiana: effects of light source on leaf temperature and hemagglutinin content. Plant Cell Rep 36, 1667–1669 (2017). https://doi.org/10.1007/s00299-017-2164-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00299-017-2164-6