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Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis

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Abstract

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We identified three physical positions associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis. We also confirmed their genetic effects on the embryo yield.

Abstract

Isolated microspore culture is well utilized for the production of haploid or doubled-haploid plants in Brassica crops. Brassica rapa cv. ‘Ho Mei’ is one of the most excellent cultivars in embryo yield of microspore culture. To identify the loci associated with microspore embryogenesis, segregation analysis of 154 DNA markers anchored to B. rapa chromosomes (A01–A10) was performed using a population of microspore-derived embryos obtained from an F1 hybrid between ‘CR-Seiga’, a low yield cultivar in microspore-derived embryos, and ‘Ho Mei’. Three regions showing significant segregation distortion with increasing ‘Ho Mei’ alleles were detected on A05, A08 and A09, although these regions showed the expected Mendelian segregation ratio in an F2 population. The additive effect of alleles in these regions on embryo yield was confirmed in a BC3F1 population. One region on A08 containing Br071-5c had a higher effect than the other regions. Polymorphism of nucleotide sequences around the Br071-5c locus was investigated to find the gene possibly responsible for efficient embryogenesis from microspores.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (B) (No. 18380003) from the Japan Society for the Promotion of Science (JSPS) and the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

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Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori-Amamiyamachi, Sendai, Miyagi, 981-8555, Japan

    Hiroyasu Kitashiba, Kumiko Taguchi, Ikuyo Kaneko, Kiyofumi Inaba & Takeshi Nishio

  2. Faculty of Agriculture, Iwate University, Morioka, Japan

    Shuji Yokoi & Yoshihito Takahata

  3. Graduate School of Life and Environmental Science, Osaka Prefecture University, Sakai, Japan

    Shuji Yokoi

Authors
  1. Hiroyasu Kitashiba
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  2. Kumiko Taguchi
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  3. Ikuyo Kaneko
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  4. Kiyofumi Inaba
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  5. Shuji Yokoi
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  6. Yoshihito Takahata
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  7. Takeshi Nishio
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Correspondence to Hiroyasu Kitashiba.

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Communicated by K. Toriyama.

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Kitashiba, H., Taguchi, K., Kaneko, I. et al. Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis. Plant Cell Rep 35, 2197–2204 (2016). https://doi.org/10.1007/s00299-016-2029-4

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  • DOI: https://doi.org/10.1007/s00299-016-2029-4

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