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Ectopic expression of Pokkali phosphoglycerate kinase-2 (OsPGK2-P) improves yield in tobacco plants under salinity stress

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Abstract

Key message

Our results indicate that OsPGK2a-P gene is differentially regulated in contrasting rice cultivars under stress and its overexpression confers salt stress tolerance in transgenic tobacco.

Abstract

Phosphoglycerate kinase (PGK; EC = 2.7.2.3) plays a major role for ATP production during glycolysis and 1, 3-bisphosphoglycerate production to participate in the Calvin cycle for carbon fixation in plants. Whole genome analysis of rice reveals the presence of four PGK genes (OsPgks) on different chromosomes. Comparative expression analysis of OsPgks in rice revealed highest level of transcripts for OsPgk2 at most of its developmental stages. Detailed characterization of OsPgk2 transcript and protein showed that it is strongly induced by salinity stress in two contrasting genotypes of rice, i.e., cv IR64 (salt sensitive) and landrace Pokkali (salt tolerant). Ectopic expression of OsPgk2a-P (isolated from Pokkali) in transgenic tobacco improved its salinity stress tolerance by higher chlorophyll retention and enhanced proline accumulation, besides maintaining better ion homeostasis. Ectopically expressing OsPgk2a-P transgenic tobacco plants showed tall phenotype with more number of pods than wild-type plants. Therefore, OsPgk2a-P appears to be a potential candidate for increasing salinity stress tolerance and enhanced yield in crop plants.

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Abbreviations

Chl:

Chloroplastic

Cyt:

Cytoplasmic

GA:

Gallic acid

PCR:

Polymerase chain reaction

PGK2:

Phosphoglycerate kinase protein 2

PGK2a-P:

PGK2a gene from rice landrace Pokkali

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

VC:

Vector control

WT:

Wild type

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Acknowledgments

Work carried out in this paper has been supported by funds available from UGC Resource Networking, Capacity building funds from JNU, Department of Science and Technology, Department of Biotechnology, Ministry of Science and Technology, Government of India. R.K. gratefully acknowledges the award of research fellowship from UGC.

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    Authors and Affiliations

    1. Stress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

      Rohit Joshi, Ratna Karan & Ashwani Pareek

    2. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India

      Sneh L. Singla-Pareek

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    1. Rohit Joshi
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    2. Ratna Karan
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    3. Sneh L. Singla-Pareek
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    4. Ashwani Pareek
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    Correspondence to Ashwani Pareek.

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    Additional information

    Communicated by N. Sreenivasulu.

    Rohit Joshi and Ratna Karan have contributed equally to this work.

    Electronic supplementary material

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    Supplementary material 1

    Total cDNA sequence of OsPgk2 along with the deduced amino acid sequence. (TIFF 271 kb)

    Supplementary material 2

    (A) Multiple alignments of IR64 and Pokkali phosphoglycerate kinase amino acid sequence with different isoforms of OsPGK2. (B) The putative gene structure of OsPGK2a, OsPGK2b and OsPGK2c were obtained from TIGR database to show the alternate splicing event between the OsPGK2 isoforms. (TIFF 80895 kb)

    Supplementary material 3

    Expression pattern of OsPgk1, OsPgk2, OsPgk3 and OsPgk4at various developmental stages of Oryza sativa based on microarray data (www.genevestigator.com). The expression data shown here is normalized, high quality and manually curated data obtained from several hundreds of independent experiments. The stage of development of plant is also shown diagrammatically in the figure. (TIFF 25296 kb)

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    Joshi, R., Karan, R., Singla-Pareek, S.L. et al. Ectopic expression of Pokkali phosphoglycerate kinase-2 (OsPGK2-P) improves yield in tobacco plants under salinity stress. Plant Cell Rep 35, 27–41 (2016). https://doi.org/10.1007/s00299-015-1864-z

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