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The AtLRK10L1.2, Arabidopsis ortholog of wheat LRK10, is involved in ABA-mediated signaling and drought resistance

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Abstract

Key message

The loss-of-function mutants of the Arabidopsis orthologue of the wheat LRK10 gene shows ABA-insensitive and drought stress-sensitive phenotypes, suggesting that LRK10L1.2 is positively involved in ABA signaling.

Abstract

A subset of receptor-like kinases (RLKs) superfamily proteins play a key role in sensing internal and external signals. A gene encoding Arabidopsis thaliana Leaf rust 10 disease-resistance locus receptor-like protein kinase 1 (AtLRK10L1), most closely related to wheat LRK10, expresses two different transcripts, LRK10L1.1 and LRK10L1.2, using alternative promoters. The T-DNA insertion mutant, lrk10l1-2, that specifically shuts down LRK10L1.2 transcription displayed an abscisic acid (ABA)-insensitive phenotype in seed germination and seedling growth. However, the lrk10l1.2 mutant exhibited reduced tolerance to drought stress, compared with wild type, which is accompanied by alteration of stomatal apertures. The transgenic plants overexpressing full-length LRK10L1.2, which localizes to the plasma membrane (PM) complemented the phenotypes of lrk10l1-2 mutant background, while those expressing LRK10L1.2 Nu1, which switched its localization to the endoplasmic reticulum (ER) by skipping of a mini-exon, showed even higher ABA insensitivity and drought sensitivity than its mutant background. Our results suggest that ABA signaling involves the PM-localized LRK10L1.2.

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Abbreviations

ABA:

Abscisic acid

AS:

Alternative splicing

ER:

Endoplasmic reticulum

IVS:

Intervening sequence

LRK10L:

Leaf rust 10 disease-resistance locus receptor-like protein kinase-like

PM:

Plasma membrane

WT:

Wild type

YFP:

Yellow fluorescent protein

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Acknowledgments

This research was supported by a grant from the BioGreen 21 Program, Rural Development Administration of Republic of Korea (PJ00948402) to S. H. K. and (PJ00822201) to S. C. L., and the National Research Foundation (NRF) of Korea Grant funded by the Korean Government (MOE) (NRF-2011-0029568) to S. H. K. and (No. 2010-0024596) to S. H. Y.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of Biological Sciences (BK21 Program), Chung-Ang University, Seoul, 156-756, Korea

    Chae Woo Lim & Sung Chul Lee

  2. Department of Bioscience and Bioinformatics, Myongji University, Yongin, Kyeonggi-Do, 449-728, Korea

    Ki Hun Shin

  3. Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin, Kyeonggi-Do, 449-728, Korea

    Seung Hwan Yang

  4. Division of Bioscience and Bioinformatics, Department of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Yongin, Kyeonggi-Do, 449-728, Korea

    Sang Hyon Kim

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  1. Chae Woo Lim
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Correspondence to Sung Chul Lee or Sang Hyon Kim.

Additional information

Communicated by Youn-Il Park.

C. W. Lim and S. H. Yang contributed equally to this work.

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299_2014_1724_MOESM1_ESM.tif

Fig. S1 a Phenotypes of WT, lrk10l-2, and 35S-Lrk10L1.2 transgenic plants in long day growth condition. Thirty-day-old plants were photographed when the primary inflorescence stems of lrk10l1-2 are 0.5 cm long. b T-DNA insertion sites of ld insertional mutants (Salk_145773 and Salk_003047). The 5´ and 3´ UTR’s and CDS are drawn by open and closed boxes, respectively. Introns shown by solid lines. c RT-PCR analysis for the LRK10L1.2 transcript from WT (Col-0) and two T-DNA insertion mutants using the primers shown in Fig. 1a (P2 and P3). (TIFF 14020 kb)

299_2014_1724_MOESM2_ESM.tif

Fig. S2 Seedling growth of WT, mutants and transgenic plants in response to mannitol, glucose, and NaCl. The seedlings were grown in 0.5× MS containing different concentrations of mannitol and glucose, and 200 mM NaCl. The representative images were taken 7 days (for 300 mM manitol, 4 and 5 % glucose) or after 10 days (for 400 mM Mannitol, 6 % glucose, and 200 mM NaCl) after stratification. (TIFF 11031 kb)

Supplementary material 3 (PPTX 83 kb)

299_2014_1724_MOESM4_ESM.tif

Fig. S4 LRK10L1.2 expression does not affect transcription of ABA marker genes, KIN1, RD22 and RAB18. Different plant samples indicated on the left were treated and collected as in Fig. 5 for RNA extraction. Gene-specific primers for the genes indicated on the right are listed in Table. S1. LRK10L1.2, the WT background of lrk10l1-2; No4, 35S-Lrk10L1.2 No4/lrk10l1-2 (Line 7) and; Nu1, 35S-Lrk10L1.2 Nu1/lrk10l1-2 (Line14). (TIFF 9488 kb)

Supplementary material 5 (DOCX 22 kb)

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Lim, C.W., Yang, S.H., Shin, K.H. et al. The AtLRK10L1.2, Arabidopsis ortholog of wheat LRK10, is involved in ABA-mediated signaling and drought resistance. Plant Cell Rep 34, 447–455 (2015). https://doi.org/10.1007/s00299-014-1724-2

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