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Albino midrib 1, encoding a putative potassium efflux antiporter, affects chloroplast development and drought tolerance in rice

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Abstract

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Mutation of the AM1 gene causes an albino midrib phenotype and enhances tolerance to drought in rice

Abstract

K+ efflux antiporter (KEA) genes encode putative potassium efflux antiporters that are mainly located in plastid-containing organisms, ranging from lower green algae to higher flowering plants. However, little genetic evidence has been provided on the functions of KEA in chloroplast development. In this study, we isolated a rice mutant, albino midrib 1 (am1), with green- and white-variegation in the first few leaves, and albino midrib phenotype in older tissues. We found that AM1 encoded a putative KEA in chloroplast. AM1 was highly expressed in leaves, while lowly in roots. Chloroplast gene expression and proteins accumulation were affected during chlorophyll biosynthesis and photosynthesis in am1 mutants. Interestingly, AM1 was induced by salt and PEG, and am1 showed enhanced sensitivity to salinity in seed germination and increased tolerance to drought. Taken together, we concluded that KEAs were involved in chloroplast development and played important roles in drought tolerance.

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Abbreviations

Car:

Carotenoid

Chl:

Chlorophyll

KEA:

Potassium (K+) efflux antiporter

ORF:

Open reading frame

DAB:

Diaminobenzidine

GFP:

Green fluorescent protein

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31000667), National Transgenic Science and Technology Program (2011ZX08009 003), and the Major State Basic Research Development Program of China (973 Program, 2009CB8500).

Conflict of interest

The authors have declared that no conflict of interests exist.

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, Hunan, China

    Peike Sheng, Junjie Tan & Xuanming Liu

  2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Peike Sheng, Junjie Tan, Mingna Jin, Fuqing Wu, Kunneng Zhou, Weiwei Ma, Yueqin Heng, Jiulin Wang, Xiuping Guo, Xin Zhang, Zhijun Cheng & Jianmin Wan

  3. National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Linglong Liu, Chunming Wang & Jianmin Wan

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  1. Peike Sheng
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  2. Junjie Tan
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Correspondence to Jianmin Wan.

Additional information

Communicated by Jeong Sheop Shin.

P. Sheng and J. Tan contributed equally to this work.

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Sheng, P., Tan, J., Jin, M. et al. Albino midrib 1, encoding a putative potassium efflux antiporter, affects chloroplast development and drought tolerance in rice. Plant Cell Rep 33, 1581–1594 (2014). https://doi.org/10.1007/s00299-014-1639-y

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  • DOI: https://doi.org/10.1007/s00299-014-1639-y

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