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The use of plants for the production of therapeutic human peptides

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Abstract

Peptides have unique properties that make them useful drug candidates for diverse indications, including allergy, infectious disease and cancer. Some peptides are intrinsically bioactive, while others can be used to induce precise immune responses by defining a minimal immunogenic region. The limitations of peptides, such as metabolic instability, short half-life and low immunogenicity, can be addressed by strategies such as multimerization or fusion to carriers, to improve their pharmacological properties. The remaining major drawback is the cost of production using conventional chemical synthesis, which is also difficult to scale-up. Over the last 15 years, plants have been shown to produce bioactive and immunogenic peptides economically and with the potential for large-scale synthesis. The production of peptides in plants is usually achieved by the genetic fusion of the corresponding nucleotide sequence to that of a carrier protein, followed by stable nuclear or plastid transformation or transient expression using bacterial or viral vectors. Chimeric plant viruses or virus-like particles can also be used to display peptide antigens, allowing the production of polyvalent vaccine candidates. Here we review progress in the field of plant-derived peptides over the last 5 years, addressing new challenges for diverse pathologies.

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Acknowledgments

This review was supported by the COST action ‘Molecular pharming: Plants as a Production Platform for High Value Proteins’ FA0804.

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Authors and Affiliations

  1. Laboratorio di Biotecnologie, Unità Tecnica BIORAD, ENEA C.R. Casaccia, 00123, Rome, Italy

    Chiara Lico

  2. Department of Agriculture, Forests, Nature and Energy (D.A.F.N.E.), University of Tuscia, Via San Camillo de Lelliss, 01100, Viterbo, Italy

    Luca Santi

  3. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    Richard M. Twyman

  4. Dipartimento di Biotecnologie, Università degli Studi di Verona, Strada Le Grazie, 15, 37134, Verona, Italy

    Mario Pezzotti & Linda Avesani

Authors
  1. Chiara Lico
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  2. Luca Santi
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  3. Richard M. Twyman
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  4. Mario Pezzotti
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  5. Linda Avesani
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Corresponding author

Correspondence to Linda Avesani.

Additional information

Communicated by R. Reski.

A contribution to the Special Issue: Plant Molecular Pharming in 2012 and Beyond.

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Lico, C., Santi, L., Twyman, R.M. et al. The use of plants for the production of therapeutic human peptides. Plant Cell Rep 31, 439–451 (2012). https://doi.org/10.1007/s00299-011-1215-7

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  • DOI: https://doi.org/10.1007/s00299-011-1215-7

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