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Auxin pulse treatment holds the potential to enhance efficiency and practicability of somatic embryogenesis in potato

  • Cell Biology and Morphogenesis
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Abstract

The objective of the current study was to simplify existing somatic embryogenesis systems in potato (Solanum tuberosum L.) cv. Desiree. The project targeted the agar-based induction phase of the potato somatic embryogenesis process as the key area for improvement. Experiments were established to ascertain the effect of a 2,4-D (2,4 dichlorophenoxyacetic acid) pulse, applied to the primary internodal section explant source and its subsequent effect on embryo induction. Parameters tested were the duration of the auxin pulse in a range from 0 to 300 min, and the concentrations of 2,4-D applied, in a range from 0 to 5,120 μM. The mean number of somatic embryos formed per explant was recorded after 4 and 8 weeks culture. Our findings indicated that the somatic embryogenesis in potato internodal segments could be evoked by an auxin (2,4-D) pulse treatment over a wide concentration and duration range. The results further suggested that a simple 20 μM 2,4-D pulse treatment could replace a lengthy 2 week induction phase in potato somatic embryogenesis and thus improve the system’s practicability for wider uptake.

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Abbreviations

2,4-D:

2,4 Dichlorophenoxyacetic acid

cDNA:

Complementary DNA

GA3 :

Gibberellic acid

IAA:

Indole acetic acid

MS:

Murashige and Skoog

NAA:

Naphthalene acetic acid

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Acknowledgment

SKS is grateful to the Government of India and the Commonwealth Scholarship Commission, UK for his doctoral Commonwealth Scholarship Award. SCRI is supported by grant-in-aid from the Scottish Executive Environment & Rural Affairs Department (SEERAD).

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Authors and Affiliations

  1. Genetics Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    Sanjeev Kumar Sharma & Glenn J. Bryan

  2. Institute of Molecular Plant Sciences, Daniel Rutherford Building, The University of Edinburgh, The Kings Buildings, Mayfield Road, Edinburgh, EH9 3JH, UK

    Steve Millam

Authors
  1. Sanjeev Kumar Sharma
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  2. Glenn J. Bryan
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  3. Steve Millam
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Correspondence to Steve Millam.

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Communicated by W. Harwood.

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Sharma, S.K., Bryan, G.J. & Millam, S. Auxin pulse treatment holds the potential to enhance efficiency and practicability of somatic embryogenesis in potato. Plant Cell Rep 26, 945–950 (2007). https://doi.org/10.1007/s00299-007-0319-6

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  • DOI: https://doi.org/10.1007/s00299-007-0319-6

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