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Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture

  • Genetics and Genomics
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Abstract

Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P≤0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17 pg/2C (cv. ‘Tinta Barroca’ and ‘Viosinho’) to 1.26 pg/2C (cv. ‘Cabernet Sauvignon’). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content.

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Abbreviations

ACM:

Anther culture medium

BAP:

Benzylaminopurine

CRBC:

Chicken red blood cells

2,4-D:

2,4-Dichlorophenoxyacetic acid

FCM:

Flow cytometry

FL:

Linear-fluorescence light scatter

MS:

Murashige and Skoog

NOA:

β-Naphtoxyacetic acid

PI:

Propidium iodide

PVP:

Polyvinylpyrrolidone

SE:

Somatic embryogenesis

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Acknowledgements

The authors thank Prof. Jaroslav Doležel (Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Olomouc, Czech Republic) for providing the reference standards used in this study. The acquisition of the flow cytometer was supported by the FCT (project POCTI/AGR/C/11142/98). FCT supported the fellowship of João Loureiro (FCT/SFRH/BD/9003/2002).

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Authors and Affiliations

  1. Center of Genetics and Biotechnology (CGB), University of Trás-os-Montes and Alto Douro, Ap. 1013, 5001-801, Vila Real, Portugal

    F. Leal & O. Pinto-Carnide

  2. Laboratory of Biotechnology and Cytomics, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    J. Loureiro, E. Rodriguez & C. Santos

  3. Unit of Molecular Biology and Plant Biotechnology (ICAT), Ed. ICAT, Campo Grande, 1749-016, Lisboa, Portugal

    M. S. Pais

Authors
  1. F. Leal
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  2. J. Loureiro
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  3. E. Rodriguez
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  4. M. S. Pais
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  5. C. Santos
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  6. O. Pinto-Carnide
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Corresponding author

Correspondence to J. Loureiro.

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Communicated by P. Ozias-Akins

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Leal, F., Loureiro, J., Rodriguez, E. et al. Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture. Plant Cell Rep 25, 978–985 (2006). https://doi.org/10.1007/s00299-006-0162-1

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  • DOI: https://doi.org/10.1007/s00299-006-0162-1

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