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Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers

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Abstract

Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for their reproducibility and high polymorphism. Out of 124 polymerase chain reaction fragments that were scored, 120 (96.8%) were polymorphic. All the plants could be discriminated and constitute a very heterogeneous group. Five unidentified almond plants found in the region of Foz Côa (north Portugal) and wild almond (P. webbii) from Italy and Spain were also included. Four main groups of plants could be distinguished: P. dulcis cultivars; one Foz Côa plant; P. webbii; and P. persica (outgroup). The segregating Foz Côa plant may represent a feral individual or a hybrid between P. dulcis and P. webbii.

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Abbreviations

dNTP :

Deoxynucleotide triphosphate

CTAB :

Cetyltrimethylammonium bromide

ISSR :

Inter-simple sequence repeats

PCR :

Polymerase chain reaction

RAPD :

Randomly amplified polymorphic DNA

RASTM :

Regional Agricultural Services of Trás-os-Montes

TE :

Tris-EDTA buffer

UPGMA :

Unweighted pair group method with arithmetical averages

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Acknowledgements

Part of this research was supported by Praxis XXI through PhD grant BD/11323/97 provided to M.C. Martins and the research projects PRAXIS 3/3.2/HORT/2143/95 and PRAXIS/PCNA/P/BIO/72/96. Prof. Peter Lindley is gratefully acknowledged for the English revision of the manuscript. We also gratefully acknowledge Ing. Vitor Cordeiro, Ing. E. Leopoldo Ferreira, Prof. Cristina Oliveira, Prof. Angelo Godini, Prof. Rafael Socias i Company and Dr. Ignasi Batlle, for providing the plant material.

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Correspondence to M. Martins.

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Communicated by P. Puigdoménech

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Martins, M., Tenreiro, R. & Oliveira, M.M. Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers. Plant Cell Rep 22, 71–78 (2003). https://doi.org/10.1007/s00299-003-0659-9

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  • DOI: https://doi.org/10.1007/s00299-003-0659-9

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