Skip to main content

Advertisement

SpringerLink
Log in

Anti-PR3 and anti-MPO antibodies are not present in sera of patients with pulmonary tuberculosis

  • Short Communication
  • Published:
Rheumatology International Aims and scope Submit manuscript

Abstract

Anti-neutrophil cytoplasmic antibodies (ANCA) are autoantibodies directed to intracellular components of neutrophils and are present in several vasculitic syndromes. Recently, these autoantibodies have been described in other autoimmune disorders as well as in infectious diseases such as tuberculosis (TB). As there are some clinical similarities between TB and granulomatosis with polyangiitis, we searched for ANCA in a group of patients with proven TB. Patients with TB confirmed by chest X-ray and sputum bacilloscopy either before or within 30 days after beginning treatment were included in this study. Anti-MPO and anti-PR3 antibodies were studied using well-standardized ELISA kits (INOVA Diagnostics, Inc.). ANCA were also investigated by indirect immunofluorescence (IIF). Fifty TB patients (26 females, mean age 47.34 ± 17 years) were enrolled in the present study. No patient tested positive for ANCA by IIF, or anti-MPO or anti-PR3 antibodies by ELISA. Although previous studies have shown the presence of ANCA in some infectious diseases, the findings of the present study demonstrated the absence of such antibodies in TB. The discrepancy in the prevalence of ANCA in TB among different studies may be attributed to methodological factors and/or the genetic background of the studied populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Hagen EC, Daha MR, Hermans J, Andrassy K, Csernok E, Gaskin G, Lesavre P, Ludemann J, Rasmussen N, Sinico RA, Wiik A, van der Woude FJ (1998) Diagnostic value of standardized assays for anti-neutrophil cytoplasmic antibodies in idiopathic systemic vasculitis. EC/BCR project for ANCA assay standardization. Kidney Int 53(3):743–753. doi:10.1046/j.1523-1755.1998.00807.x

    Article  CAS  PubMed  Google Scholar 

  2. Rother E, Schochat T, Peter HH (1996) Antineutrophil cytoplasmic antibodies (ANCA) in rheumatoid arthritis: a prospective study. Rheumatol Int 15(6):231–237

    Article  CAS  PubMed  Google Scholar 

  3. Pradhan VD, Badakere SS, Bichile LS, Almeida AF (2004) Anti-neutrophil cytoplasmic antibodies (ANCA) in systemic lupus erythematosus: prevalence, clinical associations and correlation with other autoantibodies. J Assoc Physicians India 52:533–537

    PubMed  Google Scholar 

  4. Romas E, Paspaliaris B, d’Apice AJ, Elliott PR (1992) Autoantibodies to neutrophil cytoplasmic (ANCA) and endothelial cell surface antigens (AECA) in chronic inflammatory bowel disease. Aust N Z J Med 22(6):652–659

    Article  CAS  PubMed  Google Scholar 

  5. Pradhan V, Badakere SS, Shankar Kumar U (2004) Increased incidence of cytoplasmic ANCA (cANCA) and other autoantibodies in leprosy patients from western India. Lepr Rev 75(1):50–56

    PubMed  Google Scholar 

  6. Pradhan V, Badakere SS, Shankarkumar U, Iyer YS, Ghosh K, Karnad D (2002) Anti-neutrophil cytoplasmic antibodies (ANCA) in malaria. Indian J Malariol 39(3–4):51–59

    PubMed  Google Scholar 

  7. Medina F, Camargo A, Moreno J, Zonana-Nacach A, Aceves-Avila J, Fraga A (1998) Anti-neutrophil cytoplasmic autoantibodies in leprosy. Br J Rheumatol 37(3):270–273

    Article  CAS  PubMed  Google Scholar 

  8. Soto A, Jorgensen C, Oksman F, Noel LH, Sany J (1994) Endocarditis associated with ANCA. Clin Exp Rheumatol 12(2):203–204

    CAS  PubMed  Google Scholar 

  9. Habegger de Sorrentino A, Motta P, Iliovich E, Sorrentino AP (1997) Anti-neutrophil cytoplasmic antibodies (ANCA) in patients with symptomatic and asymptomatic HIV infection. Medicina 57(3):294–298

    CAS  PubMed  Google Scholar 

  10. Bonaci-Nikolic B, Andrejevic S, Pavlovic M, Dimcic Z, Ivanovic B, Nikolic M (2010) Prolonged infections associated with antineutrophil cytoplasmic antibodies specific to proteinase 3 and myeloperoxidase: diagnostic and therapeutic challenge. Clin Rheumatol 29(8):893–904. doi:10.1007/s10067-010-1424-4

    Article  PubMed  Google Scholar 

  11. Pradhan VD, Badakere SS, Ghosh K, Pawar AR (2004) Spectrum of anti-neutrophil cytoplasmic antibodies in patients with pulmonary tuberculosis overlaps with that of Wegener’s granulomatosis. Indian J Med Sci 58(7):283–288

    PubMed  Google Scholar 

  12. Sherkat R, Mostafavizadeh K, Zeydabadi L, Shoaei P, Rostami S (2011) Antineutrophil cytoplasmic antibodies in patients with pulmonary tuberculosis. Iran J Immunol IJI 8(1):52–57. doi:http://iji.sums.ac.ir/march2011/7thiji_vol8_no1_2011.pdf

    Google Scholar 

  13. Ghosh K, Pradhan V, Ghosh K (2008) Background noise of infection for using ANCA as a diagnostic tool for vasculitis in tropical and developing countries. Parasitol Res 102(5):1093–1095. doi:10.1007/s00436-008-0929-0

    Article  PubMed  Google Scholar 

  14. Davies DJ, Moran JE, Niall JF, Ryan GB (1982) Segmental necrotising glomerulonephritis with antineutrophil antibody: possible arbovirus aetiology? Br Med J (Clin Res Ed) 285(6342):606

    Article  CAS  Google Scholar 

  15. van der Woude FJ, Rasmussen N, Lobatto S, Wiik A, Permin H, van Es LA, van der Giessen M, van der Hem GK, The TH (1985) Autoantibodies against neutrophils and monocytes: tool for diagnosis and marker of disease activity in Wegener’s granulomatosis. Lancet 1(8426):425–429

    Article  PubMed  Google Scholar 

  16. Flores-Suarez LF, Cabiedes J, Villa AR, van der Woude FJ, Alcocer-Varela J (2003) Prevalence of antineutrophil cytoplasmic autoantibodies in patients with tuberculosis. Rheumatology (Oxford) 42(2):223–229

    Article  CAS  Google Scholar 

  17. Elkayam O, Bendayan D, Segal R, Shapira Y, Gilburd B, Reuter S, Agmon-Levin N, Shoenfeld Y (2013) The effect of anti-tuberculosis treatment on levels of anti-phospholipid and anti-neutrophil cytoplasmatic antibodies in patients with active tuberculosis. Rheumatol Int 33(4):949–953. doi:10.1007/s00296-012-2487-0

    Article  CAS  PubMed  Google Scholar 

  18. Teixeira L, Mahr A, Jaureguy F, Noel LH, Nunes H, Lefort A, Barry S, Deny P, Guillevin L (2005) Low seroprevalence and poor specificity of antineutrophil cytoplasmic antibodies in tuberculosis. Rheumatology (Oxford) 44(2):247–250. doi:10.1093/rheumatology/keh467

    Article  CAS  Google Scholar 

  19. Esquivel-Valerio JA, Flores-Suarez LF, Rodriguez-Amado J, Garza-Elizondo MA, Rendon A, Salinas-Carmona MC (2010) Antineutrophil cytoplasm autoantibodies in patients with tuberculosis are directed against bactericidal/permeability increasing protein and are detected after treatment initiation. Clin Exp Rheumatol 28(1 Suppl 57):35–39

    PubMed  Google Scholar 

  20. Edington FL, Bacellar MO, Machado PR, Barbosa L, Reis E, Reis M, Santiago MB (2007) Anti-neutrophil cytoplasmic antibodies in leprosy. Clin Rheumatol 26(2):208–210. doi:10.1007/s10067-006-0281-7

    Article  PubMed  Google Scholar 

  21. Radice A, Bianchi L, Sinico RA (2013) Anti-neutrophil cytoplasmic autoantibodies: methodological aspects and clinical significance in systemic vasculitis. Autoimmun Rev 12(4):487–495. doi:10.1016/j.autrev.2012.08.008

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

M.B.S., A.A. and M.G.R. receive a scholarship from the “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq). We are indebted to the pneumologists of Hospital Otávio Mangabeira where the patients came from, for their assistance.

Conflict of interest

The authors have no conflict of interest that is directly relevant to the content of this manuscript.

Author information

Authors and Affiliations

  1. Escola Bahiana de Medicina e Saúde Pública, Rua Frei Henrique, 08, Nazaré, Salvador, Bahia, CEP 40.000-000, Brazil

    I. Lima & M. B. Santiago

  2. Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Brazil

    R. C. Oliveira, M. S. Cabral & A. Atta

  3. Fundação Osvaldo Cruz, Salvador, Bahia, Brazil

    S. Marchi, E. Reis, M. G. Reis & L. Barbosa

Authors
  1. I. Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Cabral
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Atta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. Marchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. Reis
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. G. Reis
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. Barbosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. B. Santiago
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. B. Santiago.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lima, I., Oliveira, R.C., Cabral, M.S. et al. Anti-PR3 and anti-MPO antibodies are not present in sera of patients with pulmonary tuberculosis. Rheumatol Int 34, 1231–1234 (2014). https://doi.org/10.1007/s00296-014-3009-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00296-014-3009-z

Keywords

Search

Navigation