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Involvement of soluble receptor activator of nuclear factor-κB ligand (sRANKL) in collagenase-induced murine osteoarthritis and human osteoarthritis

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Abstract

Joint destruction and excessive bone formation are associated with high expression of soluble receptor activator of nuclear factor-κB ligand (sRANKL). This study was undertaken to investigate the role of sRANKL in collagenase-induced osteoarthritis (CIOA) in mice and in patients with osteoarthritis (OA). The initial phase of CIOA was associated with severe proteoglycan depletion, decreased collagen density, and up-regulation of bone morphogenetic protein (BMP)-2. At the late stage of CIOA, bone remodeling was related with increased BMP2 and RANKL expression in the joints, high sRANKL, and decreased number of activated neutrophils in synovium. CIOA mice showed elevated plasma level of sRANKL but low RANKL expression on blood neutrophils. The percentage of RANKL-positive blood neutrophils was higher in patients with OA than in healthy individuals. Our data indicate that increased local and systemic levels of soluble RANKL might be indicative for OA disorders in mouse and human.

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Acknowledgments

This work was supported by Grants KT-X-1707 and BG051PO001-3.3.04/32 from the National Science Fund, Ministry of Education and Science, Bulgaria.

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Authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Immunology, Institute of Microbiology, Sofia, Bulgaria

    Petya Dimitrova, Valeriya Gyurkovska & Nina Ivanovska

  2. Clinic of Internal Diseases, National Transport Hospital, Sofia, Bulgaria

    Antoaneta Toncheva

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  1. Petya Dimitrova
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  2. Antoaneta Toncheva
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  3. Valeriya Gyurkovska
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  4. Nina Ivanovska
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Correspondence to Nina Ivanovska.

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Dimitrova, P., Toncheva, A., Gyurkovska, V. et al. Involvement of soluble receptor activator of nuclear factor-κB ligand (sRANKL) in collagenase-induced murine osteoarthritis and human osteoarthritis. Rheumatol Int 32, 1317–1325 (2012). https://doi.org/10.1007/s00296-010-1723-8

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  • DOI: https://doi.org/10.1007/s00296-010-1723-8

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