Abstract
The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. Comparison of the nucleotide sequences among the three plasmid DNAs indicated the presence of four regions with more than 86% sequence homology, suggesting the development of three plasmid DNAs from a common ancestor. A computer-based study of the pRS64-2 DNA-folding at both termini predicted hairpin loop structures. The hairpin loops consisted of the left- and right-hand terminal 113 and 105 nucleotides, respectively, and had no sequence homology. They had not undergone flip-flop inversion. The hairpins form cruciform base-paired structures.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 3 February / 1 August 1997
Rights and permissions
About this article
Cite this article
Katsura, K., Suzuki, F., Miyashita, SI. et al. The complete nucleotide sequence and characterization of the linear DNA plasmid pRS64-2 from the plant pathogenic fungus Rhizoctonia solani. Curr Genet 32, 431–435 (1997). https://doi.org/10.1007/s002940050299
Issue Date:
DOI: https://doi.org/10.1007/s002940050299