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Comparative analysis of putative pathogenesis-related gene expression in two Rhizoctonia solani pathosystems

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Abstract

Rhizoctonia solani, teleomorph Thanatephorus cucumeris, is a polyphagous necrotrophic plant pathogen of the Basidiomycete order that is split into 14 different anastomosis groups (AGs) based on hyphal interactions and host range. In this investigation, quantitative real-time PCR (qRT-PCR) techniques were used to determine potential pathogenicity factors of R. solani in the AG1-IA/rice and AG3/potato pathosystems. These factors were identified by mining for sequences of pathogen origin in a library of rice tissue infected with R. solani AG1-IA and comparing these sequences against the recently released R. solani AG3 genome. Ten genes common to both AGs and two specific to AG1-IA were selected for expression analysis by qRT-PCR. Results indicate that a number of genes are similarly expressed by AG1 and AG3 during the early stages of pathogenesis. Grouping of these pathogenicity factors based on relatedness of expression profiles suggests three key events are involved in R. solani pathogenesis: early host contact and infiltration, adjustment to the host environment, and pathogen proliferation through necrotic tissue. Further studies of the pathogenesis-associated genes identified in this project will enable more precise elucidation of the molecular mechanisms that allow for the widespread success of R. solani as a phytopathogen and allow for more targeted, effective methods of management.

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Acknowledgments

The authors express their gratitude to Melissa Jia and Dr. Fei Rubinelli for superb technical assistance with this project. United States Department of Agriculture is an equal opportunity provider and employer. This is Maine Agriculture Forestry Experiment Station publication # 3232.

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Author notes

  1. Renee Rioux

    Present address: Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Ave, Madison, WI, 53706, USA

  2. Harish Manmathan

    Present address: Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, 80523, USA

  3. Pratibha Singh

    Present address: Department of Biotechnology, TAU, Indo-Swiss Collaboration in Biotechnology (ISCB), Government of India, TERI, India Habitat Center, Lodhi Road, New Delhi, 110003, India

Authors and Affiliations

  1. School of Biology and Ecology, University of Maine, 5735 Hitchner Hall, Orono, ME, 04469-5735, USA

    Renee Rioux, Harish Manmathan, Benildo de los Reyes & Stellos Tavantzis

  2. University of Arkansas Rice Research and Extension Center, and USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, AR, 72160, USA

    Pratibha Singh & Yulin Jia

Authors
  1. Renee Rioux
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  2. Harish Manmathan
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  3. Pratibha Singh
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  4. Benildo de los Reyes
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  5. Yulin Jia
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  6. Stellos Tavantzis
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Corresponding author

Correspondence to Stellos Tavantzis.

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Communicated by U. Kueck.

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Rioux, R., Manmathan, H., Singh, P. et al. Comparative analysis of putative pathogenesis-related gene expression in two Rhizoctonia solani pathosystems. Curr Genet 57, 391–408 (2011). https://doi.org/10.1007/s00294-011-0353-3

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  • DOI: https://doi.org/10.1007/s00294-011-0353-3

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