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„Rapid on-site evaluation“ (ROSE) in der zytologischen Diagnostik von Lungen- und Mediastinalerkrankungen

Rapid on-site evaluation (ROSE) in cytological diagnostics of pulmonary and mediastinal diseases

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Zusammenfassung

Die endobronchiale ultraschallgestützte Feinnadelaspiration (EBUS-TBNA) ist ein integrativer Bestandteil der Diagnostik und der Staging-Untersuchungen maligner Tumoren der Lunge und des Mediastinums. „Rapid on-site evaluation“ (ROSE) bezeichnet ein zytomorphologisches Schnellverfahren, das während der laufenden bronchoskopischen Untersuchung durchgeführt werden kann und die sofortige („On-site“) Beurteilung von Materialmenge und -qualität sowie überwiegend auch bereits die Diagnose anhand einer Schnellfärbung innerhalb von Minuten ermöglicht. Dies reduziert die Anzahl benötigter Zweituntersuchungen aufgrund nicht geeigneten und/oder nicht ausreichenden Materials signifikant und vermindert so sowohl die Belastung der Patienten als auch die Kosten. Das gewonnene Material kann anschließend weiter als konventionelle Zytologie, alternativ auch im Dünnschichtverfahren („thin-prep“) und/oder als Paraffinblock aufgearbeitet werden und für weitere immunhistochemische und molekularbiologische Untersuchungen, wie z. B. Mutationsanalyse oder Durchflusszytometrie, herangezogen werden.

Abstract

Endobronchial ultrasound-guided transbronchial fine needle aspiration (EBUS-TBNA) has become an important tool in the diagnosis and staging of malignant tumors of the lungs and mediastinum. Rapid on-site evaluation (ROSE) denotes a cytomorphological diagnostic procedure that allows assessment of the adequacy and accuracy of the material obtained during bronchoscopy within a few minutes in or near the bronchoscopy suite (on-site) using a quick staining of smears. This results in a significant decrease in the number of repeated bronchoscopy procedures required to recover an adequate biopsy sample and is therefore both time and cost effective. The obtained material can be further assessed as conventional cytological specimens or alternatively using the thin-prep technique for definitive cytopathology diagnosis and/or embedded in paraffin for immunohistochemical or molecular analyses such as DNA sequencing or flow cytometry.

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Authors and Affiliations

  1. Institut für Pathologie und Neuropathologie, Universitätsklinikum Essen, Universität Duisburg-Essen, Hufelandstr. 55, 45147, Essen, Deutschland

    J. Wohlschläger, S. Ting, T. Hager, K.W. Schmid & D. Theegarten

  2. Abteilung für interventionelle Pneumologie, Ruhrlandklinik Essen, Westdeutsches Lungenzentrum am Universitätsklinikum Essen, Universität Duisburg-Essen, Essen, Deutschland

    K. Darwiche & L. Freitag

  3. Institut für Diagnostische und Interventionelle Radiologie, Universitätsklinikum Essen, Universität Duisburg-Essen, Essen, Deutschland

    H. Kühl

Authors
  1. J. Wohlschläger
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  2. K. Darwiche
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  3. S. Ting
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  4. T. Hager
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  5. L. Freitag
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  6. K.W. Schmid
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  7. H. Kühl
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  8. D. Theegarten
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Correspondence to J. Wohlschläger.

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Wohlschläger, J., Darwiche, K., Ting, S. et al. „Rapid on-site evaluation“ (ROSE) in der zytologischen Diagnostik von Lungen- und Mediastinalerkrankungen. Pathologe 33, 308–315 (2012). https://doi.org/10.1007/s00292-012-1578-8

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  • DOI: https://doi.org/10.1007/s00292-012-1578-8

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