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5-Fluorouracil and curcumin co-encapsulated chitosan/reduced graphene oxide nanocomposites against human colon cancer cell lines

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Abstract

Recent attention on chemotherapy against cancer is to explore the effective therapy through targeted delivery of anticancer agents to the tumor site by manipulating pharmacokinetic properties of nanocarriers. 5-Fluorouracil (5-FU) and curcumin (CUR) loaded chitosan/reduced graphene oxide (CS/rGO) nanocomposite has been prepared via simple chemical method. The polymer matrix-type chitosan/rGO nanocomposite, before and after encapsulation, has been analyzed by various characterizations. Entrapment and loading efficiencies were estimated. The results that demonstrated higher entrapment efficiency (> 90%) were achieved by CS/rGO nanocarrier. Various kinetic models were used to analyze the release model and to elucidate the release mechanism of the drug from CS/rGO nanocomposite. The synergistic cytotoxicity was observed on addition of 5-FU + CUR-loaded CS/rGO nanocomposite which shows the effectiveness of the system toward the inhibition of growth of HT-29 colon cancer cells. The better cytotoxicity with an IC50 of 23.8 μg/mL was observed for dual-drug-loaded nanocomposite.

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Acknowledgements

Author SD thanks UGC-UPE-Phase II for its financial aid as fellowship to carry out this research successfully. The authors would like to acknowledge Dr. J. Senthilselvan, Asst. Professor, Department of Nuclear Physics, University of Madras, for his support and helpful discussions.

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Authors and Affiliations

  1. Department of Nuclear Physics, University of Madras, Guindy Campus, Chennai, 600025, India

    S. Dhanavel, T. A. Revathy, T. Sivaranjani & A. Stephen

  2. Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600025, India

    K. Sivakumar & P. Palani

  3. Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai, 600025, India

    V. Narayanan

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  1. S. Dhanavel
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  2. T. A. Revathy
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  7. A. Stephen
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Correspondence to A. Stephen.

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Dhanavel, S., Revathy, T.A., Sivaranjani, T. et al. 5-Fluorouracil and curcumin co-encapsulated chitosan/reduced graphene oxide nanocomposites against human colon cancer cell lines. Polym. Bull. 77, 213–233 (2020). https://doi.org/10.1007/s00289-019-02734-x

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  • DOI: https://doi.org/10.1007/s00289-019-02734-x

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