Skip to main content
SpringerLink
Log in

In vivo production of neuraminidase by Pasteurella multocida A:3 in goats after transthoracic challenge

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Six goats were injected transthoracically with live Pasteurella multocida A:3 to examine if an extracellular enzyme, neuraminidase, was produced in vivo during infection with this organism. The principal group of goats (n = 6) each received 1 ml of live 7.5 × 104 cfu of P. multocida mixed with polyacrylate beads transthoracically in the left lung on day 0 and 1 ml of live P. multocida (2.2 × 108 cfu) mixed with polyacrylate beads transthoracically in the left lung on day 22. Six goats were used as negative controls and received 0.3 g of polyacrylate beads subcutaneously in the right flank on days 0 and 22. Serum was obtained from all animals on days 0, 7, 14, 22, 29, and 36. Preimmune sera from all animals showed no detectable antibody to P. multocida A:3 neuraminidase in an enzyme neutralization assay. None of the sera from the negative control animals demonstrated a significant antibody titer against the P. multocida A:3 neuraminidase. On day 36, serum samples from the six infected animals possessed complete enzyme-neutralizing activity. Anti-neuraminidase antibody could be detected as early as day 14 in the infected animals. These data show that neuraminidase is produced in vivo during an active P. multocida A:3 lobar infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Aminoff D (1961) Methods for quantitative estimation of Nacetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J 81: 384–392

    PubMed  CAS  Google Scholar 

  2. Briseldon AM, Moncla BJ, Stevens CE, Hillier SL (1992) Sialidases (neuraminidases) in bacterial vaginosis-associated microflora. J Clin Microbiol 30: 663–666

    Google Scholar 

  3. Carter GR (1967) Pasteurellosis: Pasteurella multocida and Pasteurella haemolytica. Adv Vet Sci 11: 321–379

    PubMed  CAS  Google Scholar 

  4. Consortium for Developing a Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (1988) Guide for the care and use of agricultural animals in agricultural research and teaching, Consortium for Developing a Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching, Champaign, Illinois, pp 1–74

    Google Scholar 

  5. Drzeniek R, Scharmann W, Balke E (1972) Neuraminidase and N-acetylneuraminate pyruvate-lyase of Pasteurella multocida. J Gen Microbiol 72: 357–368

    PubMed  CAS  Google Scholar 

  6. Godoy VG, Dallas MM, Russo TA, Malamy MH (1993) A role for Bacteroides fragilis neuraminidase in bacterial growth in two model systems. Infect Immun 61: 4415–4426

    PubMed  CAS  Google Scholar 

  7. Gottschalk A (1960) Correlation between composition, structure, shape, and function of a salivary mucoprotein. Nature 186: 949–951

    Article  PubMed  CAS  Google Scholar 

  8. Ifeanyi FI, Bailie WE (1992) Passive protection of mice with antiserum to neuraminidase from Pasteurella multocida. Vet Res Commun 16: 97–105

    Article  PubMed  CAS  Google Scholar 

  9. Kelly RT, Greiff D (1970) Toxicity of pneumococcal neuraminidase. Infect Immun 2: 115–117

    PubMed  CAS  Google Scholar 

  10. Kelly RT, Greiff D, Farmer S (1966) Neuraminidase activity in Diplococcus pneumoniae, J Bacteriol 91: 601–603

    PubMed  CAS  Google Scholar 

  11. Manning PJ (1984) Naturally occurring pasteurellosis in laboratory rabbits: chemical and serological studies on whole cells and lipopolysaccharides of Pasteurella multocida. Infect Immun 44: 502–507

    PubMed  CAS  Google Scholar 

  12. O’Toole RD, Goode L, Howe C (1971) Neuraminidase activity in bacterial meningitis. J Clin Invest 50: 979–985

    Article  PubMed  Google Scholar 

  13. Pardee GI (1974) The inducible neuraminidases of pathogenic microorganisms. Behring Inst Mitt 55: 103–122

    Google Scholar 

  14. Scharmann W, Drzeniek R, Blobel H (1970) Neuraminidase of Pasteurella multocida. Infect Immun 1: 319–320

    PubMed  CAS  Google Scholar 

  15. Straus DC, Purdy CW (1994) In vivo production of neuraminidase by Pasteurella haemolytica A1 in goats after transthoracic challenge. Infect Immun 62: 4675–4678

    PubMed  CAS  Google Scholar 

  16. Straus DC, Purdy CW (1995) Extracellular neuraminidase production by Pasteurella species isolated from infected animals. Curr Microbiol 31: 312–315

    Article  PubMed  CAS  Google Scholar 

  17. Straus DC, Unbehagen PJ, Purdy CW (1993) Neuraminidase production by a Pasteurella haemolytica A1 strain associated with bovine pneumonia. Infect Immun 61: 253–259

    PubMed  CAS  Google Scholar 

  18. Straus DC, Jolley WL, Purdy CW (1996) Characterization of neuraminidases produced by various serotypes of Pasteurella multocida. Infect Immun 64: 1446–1449

    PubMed  CAS  Google Scholar 

  19. White DJ, Jolley WL, Purdy CW, Straus DC (1995) Extracellular neuraminidase production by a Pasteurella multocida A:3 strain associated with bovine pneumonia. Infect Immun 63: 1703–1709

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 79430, Lubbock, TX, USA

    David C. Straus & J. Danny Cooley

  2. Conservation and Production Research Laboratory, USDA Agricultural Research Service, 79012, Bushland, TX, USA

    Charles W. Purdy

Authors
  1. David C. Straus
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Danny Cooley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Charles W. Purdy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Straus, D.C., Cooley, J.D. & Purdy, C.W. In vivo production of neuraminidase by Pasteurella multocida A:3 in goats after transthoracic challenge. Current Microbiology 33, 266–269 (1996). https://doi.org/10.1007/s002849900111

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s002849900111

Keywords

Search

Navigation