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Cloning of a New Bacillus thuringiensis cry1I-Type CrystalProtein Gene

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Abstract

A new cry1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea.

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Authors and Affiliations

  1. Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea, , , , , , KP

    Soo-Keun Choi, Byung-Sik Shin & Eun-Mee Kong

  2. Genome Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Republic of Korea, , , , , , KR

    Seung-Hwan Park

  3. Department of Molecular Biology and Research Center for Cell Differentiation, Seoul National University, Seoul, 151-742, Republic of Korea, , , , , , KR

    Hyune Mo Rho

Authors
  1. Soo-Keun Choi
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  2. Byung-Sik Shin
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  3. Eun-Mee Kong
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  4. Hyune Mo Rho
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  5. Seung-Hwan Park
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Received: 19 January 2000 / Accepted: 22 February 2000

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Choi, SK., Shin, BS., Kong, EM. et al. Cloning of a New Bacillus thuringiensis cry1I-Type CrystalProtein Gene. Curr Microbiol 41, 65–69 (2000). https://doi.org/10.1007/s002840010093

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  • DOI: https://doi.org/10.1007/s002840010093

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