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Nakazawaea tricholomae f.a., sp. nov., a Novel Ascomycetous Yeast Species Isolated from Two Mushroom Species in China

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Abstract

Two yeast strains designated as 20–27–1 and 20–28 were isolated from the fruiting bodies of Tricholoma gambosum and Marasmius maximus, respectively, which were collected in Wudaogou, Weichang county, Chengde area, Hebei Province, China. The multi-locus analysis of the sequences of the rDNA ITS, D1/D2 LSU, and SSU regions, together with partial sequences of two protein-coding genes RPB1 and TEF1 indicates that the two strains are closely related to Nakazawaea ernobii and Nakazawaea holstii, showing the similarity values of 99.3–98.7%, 97.2–97.1%, 91.9–92.5%, and 84.6% in D1/D2 LSU, ITS, TEF1, and RPB1, respectively. Physiologically, the two strains are different from N. ernobii and N. holstii in the assimilation of melibiose, inulin, and DL-lactic acid. Both the phenotypic and phylogenetic analyses indicate that those two strains represent a novel species in the genus Nakazawaea, for which the name Nakazawaea tricholomae f.a., sp. nov. (Fungal Names: FN 571492) is proposed.

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Acknowledgements

We thank Drs. Long Wang and Andrey Yurkov for critically improving the language of the manuscript.

Funding

This study was supported by grants No. 31770018 and No. 319611330200 from the National Natural Science Foundation of China (NSFC), No. 2021FY100900 from the Ministry of Science and Technology of China, No. 521000981388 from Advanced Talents Incubation Program of the Hebei University. The authors are solely responsible for the content of this work.

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Authors and Affiliations

  1. School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, 071002, Hebei, China

    Min Liu, You-Liang Jiang, Yu-Xuan Zhang & Qi-Ming Wang

  2. Hebei Innovation Center for Bioengineering and Biotechnology, Hebei University, Baoding, 071002, Hebei, China

    Min Liu

  3. Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Hebei University, Baoding, 071002, Hebei, China

    Qi-Ming Wang

  4. Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Hebei University, Baoding, 071002, Hebei, China

    Qi-Ming Wang

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  1. Min Liu
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Contributions

QMW conceived and designed the project. M.L. performed sampling and yeast isolation, Y.L.J. and Y.X.Z. performed morphological characterization and supplied various DNA sequence data. Q.M.W. and M.L. wrote and revised the paper.

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Correspondence to Qi-Ming Wang.

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Liu, M., Jiang, YL., Zhang, YX. et al. Nakazawaea tricholomae f.a., sp. nov., a Novel Ascomycetous Yeast Species Isolated from Two Mushroom Species in China. Curr Microbiol 81, 78 (2024). https://doi.org/10.1007/s00284-023-03600-w

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