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Actinomycetospora lemnae sp. nov., A Novel Actinobacterium Isolated from Lemna aequinoctialis Able to Enhance Duckweed Growth

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Abstract

Duckweed-associated actinobacteria are co-existing microbes that affect duckweed growth and adaptation. In this study, we aimed to report a novel actinobacterium species and explore its ability to enhance duckweed growth. Strain DW7H6T was isolated from duckweed, Lemna aequinoctialis. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that the strain was most closely related to Actinomycetospora straminea IY07-55T (99.0%), Actinomycetospora chibensis TT04-21T (98.9%), Actinomycetospora lutea TT00-04T (98.8%) and Actinomycetospora callitridis CAP 335T (98.4%). Chemotaxonomic and morphological characteristics of strain DW7H6T were consistent with members of the genus Actinomycetospora, while average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between the draft genomes of this strain and its closely related type strains were below the proposed threshold values used for species discrimination. Based on chemotaxonomic, phylogenetic, phenotypic, and genomic evidence obtained, we describe a novel Actinomycetospora species, for which the name Actinomycetospora lemnae sp. nov. is proposed. The type strain is DW7H6T (TBRC 15165T, NBRC 115294T). Additionally, the duckweed-associated actinobacterium strain DW7H6T was able to enhance duckweed growth when compared to the control, in which the number of fronds and biomass dry weight were increased by up to 1.4 and 1.3 fold, respectively. Moreover, several plant-associated gene features in the genome of strain DW7H6T potentially involved in plant-microbe interactions were identified.

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA-DNA hybridization

GGDC:

Genome-to-Genome Distance Calculator

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Acknowledgements

We thank Professor Aharon Oren for his kind advice on naming the species. We also acknowledge a support from Science and Technology Research Partnership for Sustainable Development (SATREPS), JICA.

Funding

This work was financially supported by Kasetsart University Research and Development Institute (KURDI) under the project FF(KU)4.64.

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  1. Yuparat Saimee

    Present address: Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, Suita, Japan

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Yuparat Saimee, Waranya Butdee, Chanita Boonmak & Kannika Duangmal

  2. Biodiversity Center Kasetsart University (BDCKU), Bangkok, 10900, Thailand

    Chanita Boonmak & Kannika Duangmal

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Contributions

Yuparat Saimee: conceptualization, performed the experiment, formal analysis, writing–original draft; Waranya Butdee: performed partial experiment; Chanita Boonmak: critical review of manuscript; Kannika Duangmal: conceptualization, writing–review and editing, supervision, project administration.

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Correspondence to Kannika Duangmal.

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The GenBank accession number for the 16S rRNA gene sequence of strain DW7H6T is OQ415442. The genome sequences of strain DW7H6T, Actinomycetospora straminae JCM 17983T, Actinomycetospora chibensis JCM 17987T, Actinomycetospora lutea TBRC 2215T, and DSM 101857T are JAQZAO000000000, JAQZAN000000000, JAQZAM000000000, JAQZAL000000000, JAQZAK000000000, respectively.

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Saimee, Y., Butdee, W., Boonmak, C. et al. Actinomycetospora lemnae sp. nov., A Novel Actinobacterium Isolated from Lemna aequinoctialis Able to Enhance Duckweed Growth. Curr Microbiol 81, 92 (2024). https://doi.org/10.1007/s00284-023-03595-4

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