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Antimicrobial Effect of Epigallocatechin Gallate Against Shewanella putrefaciens ATCC 8071: A Study Based on Cell Membrane and Biofilm

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Abstract

The study was to evaluate the antimicrobial impacts and biofilm influences on epigallocatechin gallate (EGCG) against Shewanella putrefaciens ATCC 8071. The minimum inhibitory concentration (MIC) of EGCG on S. putrefaciens was 160 μg mL−1. The growth curve exhibited that EGCG had a good antimicrobial activity. EGCG caused damages to the bacterial cell wall and membrane based the intracellular component leakage and cell viability analysis. The damage to the membrane integrity by EGCG has been confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM shows deformation of shape, TEM shows cell membrane and wall damage, and the leakage of cytoplasmic material. The treatment with EGCG at 0.25× and 0.5× MIC resulted in decreased motility and elevated levels of oxidative stress, leading to an increase in biofilm formation. These results demonstrated that EGCG may be used as a natural preservative to reduce S. putrefaciens in fish during cold storage.

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On reasonable request, the corresponding author will provide the data used or analyzed during this investigation.

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Acknowledgements

The authors thank Dr Zhengkai Yi, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China for his critical review of this manuscript.

Funding

This research was financially supported by National Natural Science Foundation of China (grant number: 31972142), Shanghai Municipal Science and technology project to enhance the capabilities of the platform (20DZ2292200, 19DZ2284000).

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Authors and Affiliations

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai, China

    Juxin Pei, Huijie Yu, Weiqiang Qiu, Jun Mei & Jing Xie

  2. National Experimental Teaching Demonstration Center for Food Science and Engineering Shanghai Ocean University, Shanghai, China

    Weiqiang Qiu, Jun Mei & Jing Xie

  3. Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, China

    Weiqiang Qiu, Jun Mei & Jing Xie

  4. Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai, China

    Weiqiang Qiu, Jun Mei & Jing Xie

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  1. Juxin Pei
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Contributions

JP and JM: Conceptualization; JP and HY: Data curation; JP and HY: Formal analysis; JX: Funding acquisition; JP and JM: Investigation; JP, JM, WQ and JX: Methodology; JM and JX: Project administration; JP and WQ: Software; JX: Validation; JP and JM: Writing-original draft; JM and JX: Writing-review & editing.

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Correspondence to Jun Mei or Jing Xie.

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Pei, J., Yu, H., Qiu, W. et al. Antimicrobial Effect of Epigallocatechin Gallate Against Shewanella putrefaciens ATCC 8071: A Study Based on Cell Membrane and Biofilm. Curr Microbiol 79, 297 (2022). https://doi.org/10.1007/s00284-022-02978-3

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