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Genotyping of Burkholderia pseudomallei Isolated From Patients in South-Western Coastal Region of India

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Abstract

Burkholderia pseudomallei is the causative agent of melioidosis. Various tools have been used to determine the genetic diversity in B. pseudomallei isolates. In this study, Random Amplified Polymorphic DNA (RAPD)—PCR and flagellin gene (fliC) based PCR-Restriction Fragment Length Polymorphism (RFLP) were used to genotype Indian clinical B. pseudomallei isolates. A total of 89 clinical isolates could be grouped in 6 groups (A through F) by RAPD-PCR analysis. Some of the isolates in various groups had identical banding pattern suggesting them to be epidemiologically related. The RAPD groups also correlated with MLST sequence types suggesting the utility of this easy to do typing method. The PCR- RFLP analysis suggested Type III to be the predominant type which is different from other RFLP types reported from Southeast Asia. In conclusion, the results of this study show that RAPD-PCR, a simple genotyping method, may be used for analyzing the B. pseudomallei isolates and also establish epidemiological relevant relatedness among them. The results of fliC PCR–RFLP further suggest the Indian isolates are different from other Southeast Asian isolates.

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Data Availability

All relevant data are available in the Manuscript.

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Acknowledgements

Authors are thankful to Director, Defence Research & Development Establishment (DRDE), Ministry of Defence, Government of India, Jhansi Road, Gwalior for his constant support and providing necessary facilities for this study. DRDE accession number for this manuscript is DRDE/MB/15/2021.

Funding

The funding for the work was met through core institutional funds. No specific funding was received for this research work.

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Authors and Affiliations

  1. Microbiology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior, 474002, Madhya Pradesh, India

    Aayushi Kamthan & Subodh Kumar

  2. Department of Microbiology, Kasturba Medical College, Manipal University, Manipal, Karnataka, India

    Chiranjay Mukhopadhyay

  3. Center for Emerging and Tropical Diseases, Manipal Academy of Higher Education, Manipal, Karnataka, India

    Chiranjay Mukhopadhyay

Authors
  1. Aayushi Kamthan
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  2. Chiranjay Mukhopadhyay
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  3. Subodh Kumar
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Contributions

SK: Research conceptualisation, data analysis, project administration, editing and review of the manuscript; CM: Research conceptualization, resources, review of the manuscript; AK: methodology and research work, draft of manuscript.

Corresponding author

Correspondence to Subodh Kumar.

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Authors have no conflict of interest.

Ethical Approval

The clinical samples were received at Department of Microbiology, Kasturba Medical College, Manipal for laboratory diagnosis and the study was approved by Institutional Ethical committee (Approval No. IEC 241/2011). The study on B. pseudomallei isolates was approved by Institutional Biosafety Committee of DRDE, Gwalior.

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Kamthan, A., Mukhopadhyay, C. & Kumar, S. Genotyping of Burkholderia pseudomallei Isolated From Patients in South-Western Coastal Region of India. Curr Microbiol 79, 226 (2022). https://doi.org/10.1007/s00284-022-02905-6

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