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Mutation of rpoS is Beneficial for Suppressing Organic Acid Secretion During 1,3-Propandiol Biosynthesis in Klebsiella pneumoniae

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Abstract

In this study, to reduce the formation of organic acid during 1,3-propanediol biosynthesis in Klebsiella pneumoniae, a method combining UV mutagenesis and high-throughput screening with pH color plates was employed to obtain K. pneumoniae mutants. When compared with the parent strain, the total organic acid formation by the mutant decreased, whereas 1,3-propanediol biosynthesis increased after 24 h anaerobic shake flask culture. Subsequently, genetic changes in the mutant were analyzed by whole-genome sequencing and verified by signal gene deletion. Mutation of the rpoS gene was confirmed to contribute to the regulation of organic acid synthesis in K. pneumoniae. Besides, rpoS deletion eliminated the formation of 2,3-butanediol, the main byproduct produced during 1,3-propanediol fermentation, indicating the role of rpoS in metabolic regulation in K. pneumoniae. Thus, a K. pneumoniae mutant was developed, which could produce lower organic acid during 1,3-propanediol fermentation due to an rpoS mutation in this study.

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Abbreviations

1,3-PD:

1,3-Propanediol

2,3-BD:

2, 3-Butanediol

Ace:

Acetate

Suc:

Succinate

3-HP:

3-Hydroxypropionic acid

Pyr:

Pyruvate

α-KG:

α-Ketoglutarate

HPLC:

High-performance liquid chromatography

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Acknowledgements

The work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Shufan Dong, Xuxia Liu, Tianyu Chen, Xiaoqin Zhou, Shengming Li, Shuilin Fu & Heng Gong

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  1. Shufan Dong
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  2. Xuxia Liu
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  3. Tianyu Chen
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  4. Xiaoqin Zhou
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  7. Heng Gong
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Contributions

SD carried out the experimental work, statistical analysis, and drafting of the manuscript. K. pneumoniae KG2 strain was isolated by TC, SL, and XL, XZ participated in the design of the experiment. HG contributed to the design and supervision of the study and also the editing of the manuscript. All the authors read and approved the manuscript.

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Correspondence to Heng Gong.

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Dong, S., Liu, X., Chen, T. et al. Mutation of rpoS is Beneficial for Suppressing Organic Acid Secretion During 1,3-Propandiol Biosynthesis in Klebsiella pneumoniae. Curr Microbiol 79, 218 (2022). https://doi.org/10.1007/s00284-022-02901-w

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